Iowa Black FQ dT Phosphoramidite

Name: Iowa Black FQ dT Phosphoramidite
Brand: BOC Sciences
Rating: 5 (1 reviews)

Catalog Number	R10-0049
Category	Other Quencher
Molecular Formula	C70H83N10O13P
Molecular Weight	1303.46

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Product Introduction

Iowa Black FQ dT Phosphoramidite is a dark quencher reagent used in oligonucleotide synthesis. It contains an Iowa Black FQ non-fluorescent chromophore attached to a thymidine (dT) phosphoramidite, enabling site-specific incorporation within the oligonucleotide sequence. It efficiently quenches fluorescence in the 420-620 nm range via Förster resonance energy transfer (FRET), making it ideal for dual-labeled probe synthesis such as TaqMan probes, molecular beacons, and other real-time PCR applications.

Chemical Information

Chemical Information

Synonyms	4-(Methyl(4-((E)-(4-nitronaphthalen-1-yl)diazenyl)phenyl)amino)butyl (6-((E)-3-(1-((2R,4S,5R)-5-((bis(4-methoxyphenyl)(phenyl)methoxy)methyl)-4-(((2-cyanoethoxy)(diisopropylamino)phosphaneyl)oxy)tetrahydrofuran-2-yl)-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)acrylamido)hexyl)carbamate
SMILES	CC(C)N(C(C)C)P(OCCC#N)O[C@H]1C[C@H](N2C=C(/C=C/C(NCCCCCCNC(OCCCCN(C)C3=CC=C(/N=N/C4=C(C=CC=C5)C5=C([N+]([O-])=O)C=C4)C=C3)=O)=O)C(NC2=O)=O)O[C@@H]1COC(C6=CC=C(OC)C=C6)(C7=CC=CC=C7)C8=CC=C(OC)C=C8
InChI	InChI=1S/C70H83N10O13P/c1-49(2)79(50(3)4)94(91-45-19-40-71)93-63-46-66(92-64(63)48-90-70(52-20-11-10-12-21-52,53-25-33-57(87-6)34-26-53)54-27-35-58(88-7)36-28-54)78-47-51(67(82)74-68(78)83)24-39-65(81)72-41-15-8-9-16-42-73-69(84)89-44-18-17-43-77(5)56-31-29-55(30-32-56)75-76-61-37-38-62(80(85)86)60-23-14-13-22-59(60)61/h10-14,20-39,47,49-50,63-64,66H,8-9,15-19,41-46,48H2,1-7H3,(H,72,81)(H,73,84)(H,74,82,83)/b39-24+,76-75+/t63-,64+,66+,94?/m0/s1
InChIKey	VPOWPZMCJKUMTC-RMDGAKQFSA-N