
FFN246 | CAS 2210244-83-8
| Catalog Number | A18-0108 |
| Category | Fluorescent Enzyme Substrates |
| Molecular Formula | C15H13FN2O |
| Molecular Weight | 256.27 |
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Product Introduction
FFN246 is a fluorescent substrate for the serotonin transporter and the vesicular monoamine transporter 2 (VMAT2).
Chemical Information
Product Specification
Application
Chemical Information
| Purity | >98% |
| IUPAC Name | 7-(2-aminoethyl)-1-fluoro-10H-acridin-9-one |
| SMILES | C1=CC2=C(C(=C1)F)C(=O)C3=C(N2)C=CC(=C3)CCN |
| InChI | InChI=1S/C15H13FN2O/c16-11-2-1-3-13-14(11)15(19)10-8-9(6-7-17)4-5-12(10)18-13/h1-5,8H,6-7,17H2,(H,18,19) |
| InChIKey | SMTKPXKSQXDLCY-UHFFFAOYSA-N |
| Solubility | Soluble in DMSO. |
| Appearance | Solid Powder |
Product Specification
| Storage | Store at -20°C |
Application
FFN246 is a fluorescent labeling reagent designed for chemical biology workflows where controlled dye conjugation is required. In typical reagent development and biomolecule labeling pipelines, FFN246 is used to introduce a fluorophore handle that enables visualization and quantitative fluorescence readouts in microscopy and fluorescence-based assays. Its practical value lies in its compatibility with established conjugation strategies used to generate fluorescent conjugates for cellular and molecular imaging experiments.
1. Fluorescent Biomolecule Labeling
FFN246 is used by chemical biology and bioconjugation researchers to prepare fluorescently labeled biomolecules for downstream imaging and binding studies. In protein, peptide, and other macromolecule labeling workflows, the reagent is incorporated to generate fluorescent conjugates that can be tracked during sample preparation, purification, and assay development. This approach is especially common when a stable fluorescent tag is needed to follow biomolecule distribution, immobilization behavior, or interaction patterns in fluorescence microscopy and plate-based fluorescence assays.
2. Fluorescence Microscopy Tracing
FFN246 supports fluorescence microscopy experiments where labeled targets need to be visualized with spatial resolution in fixed or prepared biological samples. Researchers use FFN246-derived conjugates to trace biomolecule localization in cells, map labeling patterns on biomaterials, or monitor fluorescent signal distribution across experimental conditions. The reagent's role is typically to provide a reliable fluorescent reporter for imaging readouts, enabling qualitative localization and quantitative analysis of fluorescence intensity in microscopy datasets.
3. Flow Cytometry Staining Development
FFN246 is applied in flow cytometry dye-conjugate development when fluorescent labeling of biomolecules or probe constructs is required for cell-associated signal readouts. In these workflows, FFN246 is incorporated into conjugates used to stain cells or to label binding reagents prior to cytometric acquisition. This enables researchers to quantify fluorescence intensity distributions across populations and to optimize staining conditions for assay reproducibility in instrument-based fluorescence measurements.
4. Fluorescent Assay Reagent Construction
FFN246 is also used to build fluorescent reagents for fluorescence-based bioassays and molecular imaging reagent development. Teams developing assay reagents incorporate FFN246 into conjugate formats that generate measurable fluorescence signals for endpoint readouts in microplate assays or for imaging-guided experiments. In practice, the reagent is valued as a fluorophore source for constructing labeled components that fit into common fluorescence workflows used in biochemical characterization and materials-associated fluorescence studies.
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