Cyanine5 amine | CAS 1807529-70-9

Cyanine5 amine is a cyanine dye bearing an amine functional group, enabling covalent attachment to biomolecules, polymers, and biomaterial surfaces for fluorescence labeling workflows. Its bright far-red emission supports multicolor fluorescence imaging and labeling strategies where reduced cellular autofluorescence is advantageous. In practice, the amine handle is used to generate dye conjugates for microscopy, flow cytometry, and fluorescence-based assay development.

1. Protein And Antibody Labeling

Cyanine5 amine is used by researchers and core facilities to prepare fluorescent protein conjugates for immunofluorescence, antibody labeling, and binding assays. The dye's amine functionality supports straightforward coupling to activated carboxyl groups on proteins or to amine-reactive labeling chemistries used in reagent development. Labeled antibodies and protein conjugates are commonly used to visualize target binding on fixed cell samples, to quantify binding in plate-based fluorescence assays, and to support multicolor staining panels where far-red signal separation from green channels is required.

2. Fluorescence Microscopy Staining

Cyanine5 amine is frequently incorporated into microscopy staining workflows to generate far-red fluorescent labeling reagents for cellular imaging and biomolecular localization studies. Lab groups use Cyanine5 amine-derived conjugates to label nucleic acid-binding proteins, extracellular targets, or surface-associated biomolecules in imaging experiments where strong emission in the red/far-red region improves contrast. The amine handle also supports dye conjugation to imaging standards and labeling reagents used in confocal and widefield fluorescence microscopy, including multichannel experiments that benefit from spectral spacing.

3. Flow Cytometry Fluorescent Conjugates

Cyanine5 amine is used in flow cytometry reagent development to produce far-red fluorescent conjugates for cell-surface and intracellular staining panels. Researchers conjugate the dye to antibodies, affinity reagents, or other targeting ligands to enable quantitative fluorescence readouts during sample acquisition. The amine functionality supports coupling strategies that integrate Cyanine5 into multicolor cytometry workflows, where far-red detection channels help reduce overlap with common fluorophores and improve panel design flexibility.

4. Polymer And Surface Functionalization

Cyanine5 amine is applied in biomaterials and materials research to create fluorescently labeled polymers, coatings, and surface-modified substrates for imaging and tracking. Investigators use the amine group to incorporate the dye into functional materials that require covalent attachment rather than simple adsorption, supporting reproducible fluorescence labeling of hydrogels, membranes, and engineered surfaces. Fluorescent material conjugates prepared from Cyanine5 amine are used for localization studies, material characterization by fluorescence microscopy, and fluorescence-based readouts in experimental assay systems where labeled surfaces provide the signal source.