CHO-Ph-CONH-PEG3-azide | CAS 1807540-88-0

CHO-Ph-CONH-PEG3-azide is a PEGylated azide-functionalized click chemistry reagent designed for strain-promoted or copper-catalyzed azide–alkyne cycloaddition workflows in chemical biology and materials research. The structure combines a reactive azide handle with a short PEG spacer that improves aqueous compatibility and helps control conjugation density in labeling and surface modification contexts. As a versatile azide building block, CHO-Ph-CONH-PEG3-azide is commonly incorporated into probe, linker, and polymer architectures where downstream attachment to alkyne-bearing partners enables modular assembly for imaging, diagnostics development, and biomaterials functionalization.

1. Protein Labeling And Probes

CHO-Ph-CONH-PEG3-azide is widely used as an azide-bearing intermediate for generating clickable protein conjugates and labeling reagents in chemical biology. Researchers introduce the azide functionality onto biomolecular targets to enable subsequent coupling to alkyne-functional dyes, affinity tags, or reporter scaffolds, supporting modular probe construction for workflow-driven assay development. The PEG3 spacer helps maintain solubility and reduces steric crowding around the reactive handle, which is particularly valuable when preparing libraries of differently labeled biomolecules or when tuning conjugation density for consistent reagent behavior across experiments.

2. Surface Functionalization For Biomaterials

CHO-Ph-CONH-PEG3-azide is employed to functionalize biomaterial surfaces and polymer matrices with azide groups for later click attachment of bioactive ligands, fluorescent reporters, or cell-interaction motifs. In biomaterials science, the reagent’s PEG-containing linker supports more uniform presentation of the azide moiety at interfaces, which can improve reproducibility when building multicomponent coatings or gradient-like functional layers. Downstream, alkyne-bearing partners can be attached to create stable, modular surface chemistries used in materials characterization, biosensing platform development, and advanced composite scaffold preparation.

3. Molecular Imaging And Reporter Assembly

CHO-Ph-CONH-PEG3-azide serves as a practical azide handle for assembling imaging and detection reagents through click-compatible coupling to alkyne-containing fluorophores, luminescent reporters, or imaging probes. Molecular imaging researchers and diagnostic reagent developers often rely on azide–alkyne conjugation to rapidly swap reporter components while keeping the targeting or scaffold portion constant, enabling efficient optimization of probe format and labeling strategy. The PEG3 spacer contributes to aqueous handling and can help maintain reporter accessibility, supporting consistent performance during probe synthesis and purification for research-grade imaging workflows.

4. Diagnostic Reagent Conjugation Platforms

CHO-Ph-CONH-PEG3-azide is used in the construction of diagnostic reagent conjugation platforms where modular assembly is required to generate multivalent binding reagents, labeling standards, or assay-compatible reagent sets. By providing a defined azide functionality, the reagent enables controlled attachment of alkyne-bearing components such as detection reporters, capture ligands, or reference materials in downstream manufacturing and research prototyping. The PEG3 linker supports stable conjugate formation and helps mitigate nonspecific interactions associated with overly hydrophobic linkers, which is advantageous when preparing multiplexed reagent panels or standardized conjugate lots for analytical development.