Azido-PEG11-azide | CAS 1392284-57-9

Azido-PEG11-azide is a PEG-based bifunctional azide reagent designed for copper-free and copper-mediated azide–alkyne click chemistry workflows. Its flexible, hydrophilic PEG spacer and terminal azide groups enable efficient installation of two clickable handles on biomolecules, surfaces, and polymeric scaffolds, supporting multivalent labeling and crosslinking strategies. This structure is particularly relevant for constructing well-defined conjugates, patterned materials, and imaging or assay reagents where controlled spacing and accessibility of reactive sites are important.

1. Multivalent Biomolecule Labeling

Azido-PEG11-azide is widely used to introduce two azide functionalities onto proteins, peptides, and nucleic-acid–associated constructs for subsequent click-based attachment of dyes, affinity tags, or reporter groups. The PEG11 linker helps maintain solubility and reduces steric congestion, which is beneficial when downstream conjugation requires high accessibility of each azide site. Researchers commonly employ Azido-PEG11-azide to generate multivalent probes with improved labeling density and more uniform conjugate architectures for analytical assays and molecular imaging development.

2. Surface Patterning And Coatings

Azido-PEG11-azide supports azide functionalization of material surfaces and thin films used in biosensing and research coatings, where dual azide termini can increase the probability of productive coupling to complementary clickable ligands. In surface modification workflows, the PEG spacer improves wetting and reduces nonspecific adsorption relative to more hydrophobic linkers, which can be advantageous for maintaining stable probe presentation. This reagent is frequently selected for immobilizing capture molecules, building layered functional coatings, and creating spatially organized clickable interfaces for downstream click attachment.

3. Polymer Crosslinking Frameworks

Azido-PEG11-azide is used as a crosslinking or network-building building block in polymer and hydrogel systems that rely on azide–alkyne click chemistry to form stable, modular linkages. By providing two azide groups separated by a PEG11 segment, it enables controlled formation of crosslinked architectures when paired with difunctional or multifunctional alkyne partners. Materials scientists often incorporate Azido-PEG11-azide to tune network connectivity, introduce reactive sites for post-functionalization, and produce scaffolds suitable for creating libraries of functional biomaterials and research platforms.

4. Imaging And Diagnostic Probe Building

Azido-PEG11-azide is a practical reagent for constructing click-ready imaging and assay probes that require defined linker lengths and multiple conjugation points. The bifunctional azide design allows sequential or orthogonal labeling strategies, enabling attachment of fluorophores, biotin/streptavidin-compatible tags, or other reporter moieties through click chemistry to generate probe sets with consistent geometry. Developers of molecular imaging reagents and diagnostic research tools use Azido-PEG11-azide to streamline probe synthesis, improve labeling flexibility, and support scalable production of click-assembled reagent panels.