Azido-PEG11-amine | CAS 1800414-71-4

Azido-PEG11-amine is a PEG-based azide building block designed for copper-free and copper-mediated click chemistry workflows, enabling efficient installation of azide handles on biomolecules, surfaces, and polymeric materials. The long, flexible PEG11 spacer improves aqueous solubility and reduces nonspecific interactions, while the terminal amine provides a versatile anchor for coupling to activated carboxyls, NHS-esters, isothiocyanates, or other electrophiles prior to downstream click conjugation. As a result, Azido-PEG11-amine is widely used to generate azide-functional probes and materials for modular bioconjugation, imaging reagents, and diagnostic research tools.

1. Bioconjugation Linker Chemistry

Azido-PEG11-amine is commonly used as an azide-containing PEG linker to introduce clickable functionality into proteins, peptides, and other macromolecular reagents. Researchers typically exploit the terminal amine to attach the PEG spacer to a target scaffold (for example through amide or thiourea-forming coupling chemistries), producing an azide-present conjugate that can subsequently be reacted with complementary cyclooctyne or strained-alkyne partners for rapid labeling. The PEG11 length helps maintain accessibility of the azide group in crowded biological or assay environments, supporting robust conjugation workflows in chemical biology and biomaterials labs.

2. Surface And Material Functionalization

Azido-PEG11-amine is well suited for functionalizing surfaces and polymeric materials where azide groups are required as modular attachment points. Materials scientists use the amine handle to immobilize the reagent onto activated substrates or to incorporate it into polymer networks, coatings, and hydrogel systems, then perform click-based coupling to introduce fluorescent tags, affinity ligands, or other reporter elements. The extended PEG spacer can reduce steric hindrance at the interface and improve water compatibility, which is particularly valuable for creating reproducible, biointerface-compatible clickable materials used in assay development and molecular probe fabrication.

3. Molecular Imaging Probe Preparation

Azido-PEG11-amine is frequently selected for preparing imaging and detection probes that require a stable azide moiety for later conjugation to imaging reporters. In molecular imaging reagent development, the amine functionality enables attachment to targeting scaffolds or carrier molecules, while the azide group serves as the reactive handle for subsequent click coupling with alkyne-bearing fluorophores or other imaging tags. The hydrophilic PEG11 spacer helps preserve probe behavior in aqueous formulations and supports consistent labeling density, which is advantageous for generating well-defined multicomponent imaging reagents used in research microscopy and analytical workflows.

4. Diagnostic Research Reagent Building

Azido-PEG11-amine is used in the construction of diagnostic research reagents where modular, orthogonal conjugation is required to assemble complex assay components. Teams developing biosensing and diagnostic assay toolkits often install Azido-PEG11-amine onto antibodies, binders, nanoparticles, or assay surfaces via the amine group, then use click chemistry to attach detection reporters such as fluorophores, enzyme substrates, or other signal-generating moieties bearing complementary click partners. The PEG11 spacer supports accessibility of the azide for efficient downstream coupling and helps mitigate nonspecific interactions that can otherwise affect assay background in sensitive analytical formats.