Azido-PEG11-[4-(5-(1-hydroxyethyl)-2-methoxy-4-nitrophenoxy)-butanamide] | CAS 2353409-89-7

Azido-PEG11-[4-(5-(1-hydroxyethyl)-2-methoxy-4-nitrophenoxy)-butanamide] is a PEGylated azide-functionalized building block designed for copper-free and/or copper-catalyzed click chemistry workflows, enabling efficient installation of the azide handle into larger molecular constructs. The long, flexible PEG spacer supports solubility and reduces steric constraints, while the aromatic nitrophenoxy and butanamide motifs make the reagent useful as a chemically defined imaging/labeling scaffold that can be carried through downstream conjugation and formulation steps. Researchers commonly select this type of azide-PEG reagent to prepare functional probes, polymer conjugates, and surface-tethered materials where controlled attachment via click chemistry is required.

1. Fluorescent Probe Conjugation

Azido-PEG11-[4-(5-(1-hydroxyethyl)-2-methoxy-4-nitrophenoxy)-butanamide] is frequently used as a labeling scaffold in probe development for fluorescence-based research assays, where the azide group provides a reliable click handle for attaching dyes, affinity tags, or secondary reporter moieties to biomolecules and synthetic ligands. The PEG spacer helps maintain probe accessibility and reduces non-specific interactions when the conjugate is incorporated into complex biological buffers or mixed macromolecular environments. In practice, this reagent is well suited for generating modular probe libraries in which the nitrophenoxy-containing core can be retained while the azide is swapped into different targeting or readout formats through subsequent click conjugation.

2. Biomolecule Labeling Workflows

Azido-PEG11-[4-(5-(1-hydroxyethyl)-2-methoxy-4-nitrophenoxy)-butanamide] supports azide-based bioconjugation strategies used to functionalize proteins, peptides, and other macromolecular reagents that require a defined attachment point. The PEG11 architecture is commonly leveraged to improve conjugate solubility and to provide a flexible linker that can reduce steric interference near the biomolecule’s binding or recognition regions. Teams developing labeling reagents for molecular biology and chemical biology toolkits often choose this azide-PEG scaffold to create consistent, click-compatible derivatives for downstream imaging, pull-down workflows, or analytical characterization.

3. Surface and Material Functionalization

Azido-PEG11-[4-(5-(1-hydroxyethyl)-2-methoxy-4-nitrophenoxy)-butanamide] is used in biomaterials and surface chemistry workflows to introduce a click-reactive azide functionality onto polymer surfaces, coatings, and functionalized materials. The long PEG spacer can improve wetting and reduce fouling on material interfaces, which is advantageous when preparing assay surfaces or reagent-immobilized platforms. By incorporating this reagent into material modification steps, researchers can later perform selective click coupling to attach capture ligands, fluorescent reporters, or other functional components with spatially and stoichiometrically controlled outcomes.

4. Molecular Imaging Reagent Building

Azido-PEG11-[4-(5-(1-hydroxyethyl)-2-methoxy-4-nitrophenoxy)-butanamide] is commonly incorporated into molecular imaging reagent development where modular click chemistry enables rapid assembly of imaging-capable constructs from a shared azide-containing scaffold. The nitrophenoxy-containing core provides a chemically defined motif that can be preserved while the azide handle is used to append complementary reporters or targeting elements via click-compatible partners. This approach supports iterative probe optimization in imaging tool development, including the creation of conjugate variants with different linker lengths, valencies, or reporter classes while maintaining a consistent PEG-mediated presentation of the scaffold.