Azido-PEG10-alcohol | CAS 877239-09-3

Azido-PEG10-alcohol is a polyethylene glycol (PEG)–functionalized small molecule bearing a terminal azide, making it a widely used handle for copper-free and copper-mediated click chemistry workflows. As a PEG-based linker, it combines the chemical reactivity of an azide with the solubility and biocompatibility advantages commonly sought in bioconjugation and materials modification. This reagent is frequently selected to introduce azide functionality onto surfaces, biomolecules, and polymer constructs for subsequent conjugation to strained alkynes, cyclooctynes, or other click partners used in chemical biology, imaging, and diagnostic reagent development.

1. Bioconjugation Linkers

Azido-PEG10-alcohol is commonly used as a PEG spacer for preparing azide-bearing bioconjugates, including labeling reagents and functionalized probes that require improved aqueous compatibility and reduced steric interference. Researchers incorporate the azide handle into biomolecule conjugation schemes to enable modular assembly with cyclooctyne/alkyne click partners, supporting reliable coupling of targeting ligands, affinity tags, or reporter motifs to proteins, peptides, and nucleic-acid reagents. The PEG10 length helps maintain accessibility of the reactive handle and can improve dispersion of conjugates in assay buffers used for downstream analytical workflows.

2. Surface And Material Functionalization

Azido-PEG10-alcohol is used to introduce azide functionality onto polymer films, hydrogel networks, and engineered material surfaces, enabling subsequent click attachment of fluorescent labels, affinity groups, or bioactive moieties. In biomaterials science, the PEG spacer is valuable for tuning surface hydration and reducing nonspecific interactions while maintaining a reactive azide site for orthogonal coupling. This approach supports the fabrication of modular sensing and research platforms where azide-functional surfaces are later decorated with complementary click reagents to create spatially addressable or batch-assembled materials.

3. Fluorescent Probe And Imaging Assembly

Azido-PEG10-alcohol serves as an azide-bearing building block for constructing fluorescent probes and molecular imaging reagents that benefit from PEG-mediated solubilization and reduced aggregation. Chemical biology teams use the azide handle to couple fluorophores, quenchers, or imaging reporters to targeting scaffolds via click-compatible partners, producing conjugates with improved handling in aqueous media. The PEG10 alcohol motif is also frequently selected when a flexible linker is needed to preserve signal reporting and to maintain effective presentation of the imaging component in complex labeling and wash steps.

4. Diagnostic Reagent Platforms

Azido-PEG10-alcohol is applied in the development of diagnostic and analytical reagent components that rely on modular click chemistry for assembling multicomponent assay constructs. Teams designing affinity-based detection reagents use azide-functional PEG linkers to attach reporters, capture ligands, or signal-amplifying modules to assay surfaces and particulate formats using click-compatible chemistries. The PEG spacer supports robust reagent preparation and consistent conjugate behavior during reagent storage, mixing, and incubation steps typical of research diagnostics and analytical assay workflows.