ATTO Rho13 NHS ester

ATTO Rho13 NHS ester is an amine-reactive ATTO dye designed for fluorescent labeling of proteins and other primary-amine containing biomolecules via NHS ester chemistry. Its rhodamine-family emission supports bright red fluorescence for microscopy and quantitative fluorescence workflows, and the activated ester format enables straightforward conjugate preparation for research-grade imaging reagents and assay standards.

1. Protein And Antibody Labeling

ATTO Rho13 NHS ester is widely used by bioconjugation groups to prepare fluorescent protein conjugates for fluorescence microscopy, immunostaining, and binding assays. Researchers typically label antibodies, antibody fragments, enzymes, and carrier proteins to generate fluorescent probes with red emission that can be tracked during incubation, washing, and imaging steps. The NHS-ester reactivity toward lysine side-chain amines supports robust conjugation for building imaging reagents, flow cytometry dyes, and assay reagents where consistent labeling is required for quantitative readouts.

2. Fluorescence Microscopy Staining

ATTO Rho13 NHS ester supports cellular and biomolecular visualization workflows where red fluorescence is advantageous for multicolor experiments and background discrimination. Imaging teams use ATTO Rho13-labeled proteins or other amine-containing targets to stain extracellular components, label binding partners on cell surfaces, or tag macromolecular assemblies in fixed-cell or in vitro imaging formats. The dye's rhodamine-like brightness makes it useful for preparing microscopy stains and fluorescent labeling reagents for confocal and widefield fluorescence imaging.

3. Flow Cytometry Fluorescent Conjugates

ATTO Rho13 NHS ester is used in flow cytometry reagent development to generate red-emitting fluorescent conjugates for cell-surface or extracellular labeling strategies based on antibody or protein recognition. Assay developers prepare ATTO Rho13-labeled affinity reagents to quantify binding signals by fluorescence readout in single-cell analysis workflows. The NHS-ester format is commonly selected when a lab needs to generate a custom fluorescent conjugate for gating strategies, comparator controls, and multicolor panels that include red-channel detection.

4. Fluorescent Bioassay Reagents

ATTO Rho13 NHS ester is frequently incorporated into fluorescence assay development where labeled proteins serve as reporters, tracers, or detection reagents. Biochemistry and chemical biology teams prepare ATTO Rho13 conjugates for binding assays, immobilized-protein workflows, and fluorescence-based characterization of biomolecular interactions. In these applications, the dye's red fluorescence supports convenient instrument compatibility with common fluorescence readers and imaging systems used for assay readouts.