ATTO 740-Streptavidin

ATTO 740-Streptavidin is a fluorescently labeled streptavidin conjugate designed for high-affinity biotin capture in fluorescence-based workflows. The ATTO 740 fluorophore provides far-red excitation/emission signal for microscopy and plate-based readouts, while the streptavidin scaffold enables robust, non-covalent attachment to biotinylated biomolecules, affinity handles, and probes. This reagent is commonly used when researchers need reliable fluorescent tagging of biotinylated targets such as antibodies, nucleic acids, peptides, or surface-immobilized ligands.

1. Biotin Capture Labeling

ATTO 740-Streptavidin is widely used to fluorescently label biotinylated proteins, peptides, and affinity reagents in biochemical binding studies. Researchers typically pre-biotinylate the target (for example, an antibody, ligand, or engineered protein domain) and then use this conjugate to generate a far-red fluorescent complex for visualization and quantification. The strong streptavidin-biotin interaction supports consistent labeling of heterogeneous samples, including mixtures where the biotinylated component is present at low abundance.

2. Fluorescence Microscopy Staining

ATTO 740-Streptavidin supports far-red fluorescence microscopy workflows where biotinylated probes are used to mark cellular or extracellular structures. In imaging experiments, this conjugate is commonly applied after probe incubation to create a fluorescent readout from biotin-tagged targeting reagents, enabling localization studies with minimal perturbation of the biotinylated construct. The far-red ATTO 740 signal is particularly useful for multiplexing with other fluorophores and for reducing spectral overlap in complex staining panels.

3. Flow Cytometry Detection

ATTO 740-Streptavidin is used in flow cytometry to generate a fluorescent signal from biotinylated labeling reagents, including biotinylated antibodies and detection probes. In typical workflows, cells or particles are incubated with a biotinylated binder, followed by staining with the ATTO 740 conjugate to report binding by fluorescence readout. This approach is frequently selected when researchers want a modular staining strategy that separates target recognition (biotinylated reagent) from signal generation (ATTO 740-Streptavidin).

4. Western Blot And Blotting

ATTO 740-Streptavidin is used as a fluorescence detection reagent for blotting workflows that involve biotinylated primary reagents or biotinylated detection systems. After transfer and blocking, biotinylated binders can be visualized by incubating with the ATTO 740 conjugate, enabling far-red fluorescence readout of protein bands or immobilized biomolecules. This format is commonly used in research settings to streamline detection when biotin-based affinity reagents are already part of the assay design.