ATTO 700-Streptavidin

ATTO 700-Streptavidin is a fluorescent streptavidin conjugate designed for strong, biotin-mediated labeling workflows in fluorescence microscopy, flow cytometry, and assay development. The ATTO 700 dye provides a far-red emission signal that is well suited for multicolor imaging experiments where spectral separation from green and orange channels is required. This reagent is commonly used by cell biology and molecular imaging groups to generate consistent fluorescent readouts from biotinylated targets, including proteins, nucleic acids, and affinity reagents.

1. Biotin-Based Fluorescent Labeling

ATTO 700-Streptavidin is used as a labeling reagent to visualize biotinylated biomolecules with high binding affinity, enabling straightforward fluorescent tagging of proteins, peptides, and other biotin-containing constructs. Researchers rely on the streptavidin scaffold to create uniform, reproducible fluorescent conjugates for imaging and quantitative fluorescence readouts, particularly when the biotinylation pattern is already established on the target of interest.

2. Fluorescence Microscopy Staining

ATTO 700-Streptavidin supports far-red fluorescence microscopy workflows where biotinylated probes are used to mark cellular components, extracellular targets, or assay-specific biomolecular assemblies. Imaging groups commonly apply this conjugate for endpoint staining and localization studies, taking advantage of the ATTO 700 emission to reduce spectral overlap with commonly used green/orange fluorophores in multichannel experiments.

3. Flow Cytometry Detection

ATTO 700-Streptavidin is frequently used in flow cytometry to quantify biotinylated ligands and labeling reagents on cell surfaces or in binding assays. Flow cytometry users benefit from the streptavidin-biotin format because it integrates cleanly with existing biotinylated antibodies, aptamers, or secondary reagents, producing a robust fluorescence signal for gating-based analysis of labeled populations.

4. Bioconjugate and Assay Reagent Building

ATTO 700-Streptavidin is also used as a fluorescent component in the construction of bioconjugates and diagnostic-style research reagents that rely on biotin capture and fluorescent readout. Chemical biology and assay development teams incorporate the conjugate into platform development for homogeneous fluorescence measurements and labeling-based detection schemes where the streptavidin binding step provides a convenient route to assemble fluorescent complexes from biotinylated building blocks.