ATTO 700 azide

ATTO 700 azide is a reactive azide click chemistry reagent designed for labeling workflows that require efficient conjugation of biomolecules, polymers, and surfaces. As an ATTO 700 fluorophore, it provides far-red fluorescence for microscopy and fluorescence-based readouts, enabling visualization of tagged targets after copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) or related azide-alkyne coupling strategies. Its azide handle makes it a convenient building block for constructing fluorescent conjugates, including probe and material labeling formats.

1. Biomolecule Labeling

ATTO 700 azide is frequently used by chemical biology and bioconjugation groups to install far-red fluorescence onto proteins, peptides, and other biomolecular constructs through azide-alkyne click labeling. Researchers commonly pair it with alkyne-functionalized biomolecules (e.g., commercially available alkyne-tagged antibodies, ligands, or affinity reagents) to generate fluorescent conjugates for tracking binding, monitoring localization, and supporting downstream imaging workflows. The azide functionality supports modular assembly, allowing the fluorophore to be introduced at the stage most convenient for conjugate preparation while maintaining compatibility with established click-labeling pipelines.

2. Fluorescence Imaging Probes

ATTO 700 azide is used in fluorescence microscopy and imaging probe development where far-red signal is advantageous for multi-color experiments and reduced background from shorter-wavelength channels. In typical probe-building workflows, the azide is incorporated into imaging reagents that are later coupled to targeting vectors or scaffold components bearing complementary alkyne groups. This approach is especially common in research settings that require robust, covalent fluorescent tagging for fixed-sample imaging, assay development, and quantitative fluorescence readouts where stable conjugation is important.

3. Surface Functionalization

ATTO 700 azide supports biomaterials and surface engineering efforts that require fluorescent surface modification for material characterization and spatial studies. Teams working on hydrogels, nanoparticles, and functional coatings often use the azide to create fluorescently labeled surfaces after click coupling to alkyne-bearing linkers or surface coatings. The resulting far-red fluorescence enables visualization of coating uniformity, tracking of material-biomolecule interactions, and preparation of fluorescently tagged materials for microscopy-based studies and fluorescence quantification in assay development.

4. Nucleic Acid Labeling

ATTO 700 azide is also applied in oligonucleotide labeling workflows where azide-alkyne click chemistry is used to attach far-red fluorophores to nucleic acid probes and related constructs. Molecular biology and nucleic-acid analytics groups use azide-functional fluorophores to generate fluorescently labeled primers, probes, or hybridization reagents that can be read by fluorescence imaging or plate-based fluorescence measurements. This labeling strategy is commonly selected when researchers want a covalent, modular fluorescent handle that can be introduced without relying on dye chemistries that may interfere with nucleic acid handling and assay workflows.