ATTO 700

ATTO 700 is a far-red fluorescent dye used as a labeling fluorophore for fluorescence imaging and quantification workflows where reduced background and deep tissue/low-autofluorescence channels are advantageous. Its strong emission in the red spectral region makes it a common choice for multicolor experiments and for tracking biomolecular conjugates in microscopy and plate-based assays, typically after covalent attachment to proteins, peptides, nucleic acids, or other targeting ligands.

1. Fluorescence Microscopy Labeling

ATTO 700 is frequently used by cell biology and imaging labs to visualize labeled biomolecules in fluorescence microscopy experiments, including fixed-sample workflows and imaging of fluorescent conjugates in cultured cells. Researchers incorporate ATTO 700 into antibody, protein, or peptide labeling strategies to generate far-red imaging reagents that can be tracked over time in standard fluorescence microscope setups. The dye's far-red emission helps separate the labeled signal from common cellular autofluorescence, supporting clearer localization readouts in multichannel imaging experiments.

2. Flow Cytometry Conjugates

ATTO 700 is also used in flow cytometry workflows as a far-red reporter dye for fluorescently labeled antibodies and other binding reagents. Flow cytometry users rely on ATTO 700-conjugated constructs to quantify binding to cell-surface targets or to monitor labeling of biomolecules associated with specific cell populations during analytical screening. In multicolor panels, ATTO 700's emission region is commonly selected to balance spectral separation across fluorophores, enabling robust gating and comparative fluorescence intensity measurements across experimental conditions.

3. Fluorescent Bioassay Tracers

ATTO 700 is commonly employed in fluorescence-based bioassay development as a tracer fluorophore for monitoring binding, uptake, or interaction events using plate readers and imaging plate formats. Assay developers label assay components such as proteins, peptides, or nucleic-acid-containing reagents with ATTO 700 to generate fluorescent readouts that can be monitored quantitatively. The far-red signal is particularly useful when assays must be performed in the presence of biological matrices that contribute to background fluorescence in shorter wavelength channels.

4. Multicolor FRET Pairing

ATTO 700 is used in fluorescence technology development to construct FRET-based systems where a far-red acceptor fluorophore is required. Researchers design donor-acceptor labeling schemes by attaching ATTO 700 to one component of a molecular interaction pair, enabling distance-dependent energy transfer readouts in solution or on biomolecular assemblies. This makes ATTO 700 relevant for assay prototyping in chemical biology and biomaterials research, including studies that use fluorescence resonance energy transfer to monitor conformational changes or molecular proximity.