![{[Name]}](https://resource.bocsci.com/structure/18997-57-4.gif?v=2025011501)
![{[Name]}](https://resource.bocsci.com/structure/206443-06-3.gif?v=2025011501)
![{[Name]}](https://resource.bocsci.com/structure/460085-45-4.gif?v=2025011501)
![{[Name]}](https://resource.bocsci.com/structure/126938-14-5.gif?v=2025011501)
![{[Name]}](https://resource.bocsci.com/structure/149695-85-2.gif?v=2025011501)
![{[Name]}](https://resource.bocsci.com/structure/3368-04-5.gif?v=2025011501)
Inquiry Basket
AMPPD | CAS 122341-56-4
| Catalog Number | A18-0070 |
| Category | Fluorescent Enzyme Substrates |
| Molecular Formula | C18H23O7P |
| Molecular Weight | 382.34 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
AMPPD is a sensitive fluorogenic substrate for alkaline phosphatase. It is mainly used for immunodetection, such as tumor markers, infectious diseases, endocrine function, hormones and other aspects of the diagnosis.
Chemical Information
Product Specification
Application
Computed Properties
Patents
| Related CAS | 124951-96-8 (disodium salt) |
| Synonyms | 3-[2-spiroadamatane]-4-methoxy-4-[3-phosphoryloxy]-phenyl-1,2-dioxetane; 3-(4'-Methoxyspiro[adamantane-2,3'-[1,2]dioxetan]-4'-yl)phenyl dihydrogen phosphate; 3-(2'-Spiroadamantane)-4-methoxy-4-(3''-phosphoryloxy)phenyl-1,2-dioxetane; 4-Methoxy-4-(3-phosphatephenyl)spiro(1,2-dioxetane)-3,2'-adamantane; 3-(4-Methoxyspiro(1,2-dioxetane-3,2'-tricyclo(3.3.1.1(3,7))decan)-4-yl)phenyl phosphate; Lumigen PPD |
| Purity | ≥98% by HPLC |
| IUPAC Name | [3-(3'-methoxyspiro[adamantane-2,4'-dioxetane]-3'-yl)phenyl] dihydrogen phosphate |
| Canonical SMILES | COC1(C2(C3CC4CC(C3)CC2C4)OO1)C5=CC(=CC=C5)OP(=O)(O)O |
| InChI | InChI=1S/C18H23O7P/c1-22-18(13-3-2-4-16(10-13)23-26(19,20)21)17(24-25-18)14-6-11-5-12(8-14)9-15(17)7-11/h2-4,10-12,14-15H,5-9H2,1H3,(H2,19,20,21) |
| InChIKey | XYIPYISRNJUPBA-UHFFFAOYSA-N |
| Solubility | Soluble in Water |
| Density | 1.47±0.1 g/cm3 (Predicted) |
| Appearance | White Powder |
| Boiling Point | 548.6±60.0°C (Predicted) |
| Storage | -20°C |
AMPPD, known as 3-(2’-spiroadamantane)-4-methoxy-4-(3’’-phosphoryloxy)phenyl-1,2-dioxetane, is a widely used chemiluminescent substrate in various bioscience applications. Here are four key applications of AMPPD:
Molecular Diagnostics: In the realm of diagnostics, AMPPD plays a pivotal role in detecting specific nucleic acids and proteins with precision. In assays like ELISA, the enzyme alkaline phosphatase catalyzes the conversion of AMPPD into a luminescent product, enabling sensitive detection. This enhanced accuracy is instrumental in diagnostic tests for diseases ranging from cancer to infectious diseases.
Western Blotting: Utilized in Western blotting, AMPPD acts as a substrate for visualizing alkaline phosphatase-labeled antibodies. The ensuing chemiluminescent reaction produces a signal directly proportional to the target protein’s abundance in the sample. This method empowers researchers to visualize and quantify specific proteins with exceptional sensitivity and accuracy.
Bioluminescent Imaging: Emerging as a cornerstone in biological research, AMPPD is integral to bioluminescent imaging techniques that offer real-time insights into cellular activities. By leveraging genetically modified cells or organisms expressing alkaline phosphatase, researchers can track disease progression, gene expression, and therapeutic responses. This real-time monitoring is invaluable for unraveling the intricate dynamics of biological systems.
Environmental Monitoring: Expanding into environmental science, AMPPD-based assays are deployed for detecting contaminants like pathogens and pollutants in water and soil samples. The chemiluminescent signal produced by AMPPD allows for precise quantification of these substances with heightened sensitivity. These rapid and accurate detection methods bolster environmental safety and public health efforts.
| XLogP3 | 1.7 |
| Hydrogen Bond Donor Count | 2 |
| Hydrogen Bond Acceptor Count | 7 |
| Rotatable Bond Count | 4 |
| Exact Mass | 382.11814007 g/mol |
| Monoisotopic Mass | 382.11814007 g/mol |
| Topological Polar Surface Area | 94.4Ų |
| Heavy Atom Count | 26 |
| Formal Charge | 0 |
| Complexity | 591 |
| Isotope Atom Count | 0 |
| Defined Atom Stereocenter Count | 0 |
| Undefined Atom Stereocenter Count | 1 |
| Defined Bond Stereocenter Count | 0 |
| Undefined Bond Stereocenter Count | 0 |
| Covalently-Bonded Unit Count | 1 |
| Compound Is Canonicalized | Yes |
| Publication Number | Title | Priority Date |
|---|---|---|
| CN-115073492-A | An olanzapine derivative antigen, olanzapine antibody and application thereof | 2022-08-22 |
| CN-115073492-B | A kind of olanzapine derivative antigen, olanzapine antibody and application thereof | 2022-08-22 |
| CN-115057871-A | Rifampicin hapten derivative, rifampicin complete antigen, rifampicin antibody and application | 2022-08-17 |
| CN-115057871-B | Rifampicin hapten derivatives, rifampicin complete antigens, rifampicin antibodies and their applications | 2022-08-17 |
| CN-115201183-A | A single dose of S100β protein chemiluminescence assay kit and assay method thereof | 2022-08-04 |
Recommended Services
Recommended Articles
- Hoechst Dyes: Definition, Structure, Mechanism and Applications
- Mastering the Spectrum: A Comprehensive Guide to Cy3 and Cy5 Dyes
- Fluorescent Probes: Definition, Structure, Types and Application
- Fluorescent Dyes: Definition, Mechanism, Types and Application
- Coumarin Dyes: Definition, Structure, Benefits, Synthesis and Uses
- BODIPY Dyes: Definition, Structure, Synthesis and Uses
- Cyanine Dyes: Definition, Structure, Types and Uses
- Fluorescein Dyes: Definition, Structure, Synthesis and Uses
- Rhodamine Dyes: Definition, Structure, Uses, Excitation and Emission
- Unlocking the Power of Fluorescence Imaging: A Comprehensive Guide
- Cell Imaging: Definitions, Systems, Protocols, Dyes, and Applications
- Lipid Staining: Definition, Principles, Methods, Dyes, and Uses
- Flow Cytometry: Definition, Principles, Protocols, Dyes, and Uses
- Nucleic Acid Staining: Definition, Principles, Dyes, Procedures, and Uses
- DNA Staining: Definition, Procedures, Benefits, Dyes and Uses
- Cell Staining: Definition, Principles, Protocols, Dyes, and Uses
- Ion Imaging: Definition, Principles, Benefits, Dyes, and Uses
- Fluorescent Labeling: Definition, Principles, Types and Applications
Recommended Products
Online Inquiry
