Aminooxy-PEG5-azide | CAS 1919045-02-5

Aminooxy-PEG5-azide is a PEG-based azide click reagent designed for bioorthogonal conjugation workflows. It couples an aminooxy functional handle with a terminal azide, enabling oxime-directed attachment to carbonyl-containing biomolecules and subsequent azide-compatible click chemistry for modular labeling. The flexible PEG5 spacer improves solubility and reduces steric constraints, making this reagent broadly useful for constructing functional bioconjugates, imaging probes, and surface or material-bound linkers.

1. Oxime-Based Biomolecule Labeling

Aminooxy-PEG5-azide is commonly used to install an azide functionality onto aldehyde- or ketone-bearing biomolecules via aminooxy/oxime chemistry, creating a versatile intermediate for downstream click labeling. Researchers use it to generate azide-tagged proteins, peptides, and glycan-modified constructs where PEG spacing helps preserve binding or recognition properties during conjugation. The resulting azide handle is then compatible with copper-free or copper-mediated azide–alkyne click strategies, supporting modular workflows in chemical biology and reagent development.

2. Fluorescent and Imaging Probe Conjugation

Aminooxy-PEG5-azide supports the preparation of fluorescent probes and molecular imaging reagents by providing a stable azide handle for attaching dyes, reporters, or imaging moieties to biomolecular targeting scaffolds. In probe development, the PEG5 linker is frequently used to improve aqueous handling and to mitigate steric effects that can otherwise reduce labeling efficiency or alter optical behavior. This reagent is therefore a practical choice for building multi-component imaging tools where orthogonal conjugation steps are required to assemble final probe architectures.

3. Surface and Biomaterial Functionalization

Aminooxy-PEG5-azide is used to functionalize biomaterials, coatings, and surfaces by first introducing oxime-forming attachment to carbonyl-present substrates, followed by azide-driven click coupling to immobilize ligands or reactive partners. Materials scientists value the PEG spacer for promoting uniform presentation of functional groups and for improving resistance to nonspecific interactions in many surface chemistry applications. The azide functionality enables rapid, modular installation of peptides, polymers, or other linkers onto engineered materials used in research-grade diagnostics development and biointerface studies.

4. Diagnostic Reagent Assembly

Aminooxy-PEG5-azide is frequently incorporated into diagnostic reagent development workflows to create azide-enabled conjugates that can be rapidly assembled into multivalent detection formats. Teams in assay and reagent engineering use the aminooxy handle to connect the PEG5-azide motif to carbonyl-bearing components, then rely on azide click compatibility to attach detection reporters or secondary binding elements. This approach is especially useful when iterative, component-wise assembly is needed to generate consistent reagent batches for assay optimization and platform prototyping.