Rhodamine 110 chloride | CAS 13558-31-1

Rhodamine 110 chloride is a xanthene-family fluorescent dye valued for its strong fluorescence in the visible range and its utility as a labeling fluorophore in chemical biology workflows. Its cationic, water-compatible form supports convenient conjugate preparation and fluorescence readouts in microscopy and spectroscopy-based assays, where rhodamine emission is frequently used to visualize labeled biomolecules and monitor reaction progress.

1. Fluorescent Labeling Probes

Rhodamine 110 chloride is frequently used as a fluorescent label for preparing conjugates for biomolecule tracking, including labeling of peptides, proteins, and nucleic-acid-related constructs in research settings. Researchers incorporate the dye into labeling workflows to generate fluorescent conjugates that can be followed by fluorescence microscopy, plate-reader assays, or gel-based fluorescence detection, enabling localization studies and quantitative readouts of labeled material. Its rhodamine emission profile makes it a common choice when a bright, visible fluorescent tag is required for routine labeling and imaging experiments.

2. Fluorescence Microscopy Imaging

Rhodamine 110 chloride is applied in fluorescence microscopy to visualize fluorescently tagged targets in fixed-cell or fixed-sample imaging experiments where rhodamine fluorescence provides robust contrast. In microscopy workflows, the dye is often used as a reporter for labeled biomolecules or as a component of staining and imaging reagents, supporting studies of sample distribution, uptake of labeled probes, and spatial mapping of fluorescent conjugates. The dye's widely used excitation/emission characteristics also align well with standard fluorescence microscope filter sets used in many life-science laboratories.

3. Flow Cytometry Fluorescent Tracing

Rhodamine 110 chloride is used in flow cytometry workflows that rely on fluorescent labeling of biomolecules or labeled probe components for cell-associated readouts. In these applications, the dye supports fluorescence-based quantification of labeled populations, such as tracking binding of fluorescent conjugates to cell-associated targets or monitoring the presence of fluorescently tagged reagents in suspension assays. Researchers typically select rhodamine-based labels like this when they need a visible-range fluorophore compatible with common cytometer configurations for routine screening and comparative fluorescence measurements.

4. Fluorescence-Based Bioassay Development

Rhodamine 110 chloride is commonly incorporated into fluorescence assay development to create fluorescent reporters for monitoring biochemical reactions and labeling steps in vitro. Assay developers use the dye to generate fluorescent standards, to track reaction progress in fluorescence readouts, and to support endpoint or kinetic measurements in microplate formats. Its role as a practical rhodamine fluorophore makes it useful for building assay workflows where a stable, bright fluorescent signal is required for quantitative comparison across experimental conditions.