5-FAM Azide, CHC protected

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5-FAM Azide, CHC protected

5-FAM Azide, CHC protected

Catalog Number F04-0056
Category Azides
Molecular Formula C38H38N4O8
Molecular Weight 678.74
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Product Introduction

5-FAM Azide, CHC protected is a fluorescent labeling reagent used for bioorthogonal click chemistry. It contains a 5-carboxyfluorescein (5-FAM) fluorophore with cyclohexylcarbonyl (CHC) protecting groups on the phenolic hydroxyl groups, conjugated to an azide functional group. The azide moiety enables copper-catalyzed or strain-promoted azide-alkyne cycloaddition with alkyne-functionalized molecules, while the CHC protection enhances stability during synthesis and handling. It is used for labeling proteins, peptides, and oligonucleotides.

  • Chemical Information

Chemical Information

Synonyms 5-((3-Azidopropyl)carbamoyl)-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl dicyclohexanecarboxylate; FAM azide, 5-isomer, CHC protected
SMILES [N-]=[N+]=NCCCNC(C1=CC=C(C23C(C=C4)=C(OC5=C2C=CC(OC(C6CCCCC6)=O)=C5)C=C4OC(C7CCCCC7)=O)C(C(O3)=O)=C1)=O
InChI InChI=1S/C38H38N4O8/c39-42-41-19-7-18-40-34(43)25-12-15-29-28(20-25)37(46)50-38(29)30-16-13-26(47-35(44)23-8-3-1-4-9-23)21-32(30)49-33-22-27(14-17-31(33)38)48-36(45)24-10-5-2-6-11-24/h12-17,20-24H,1-11,18-19H2,(H,40,43)
InChIKey FOPWQDRCRPVATJ-UHFFFAOYSA-N

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