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Ac-IETD-AFC | CAS 211990-57-7
| Catalog Number | A18-0006 |
| Category | Fluorescent Enzyme Substrates |
| Molecular Formula | C31H38F3N5O12 |
| Molecular Weight | 729.7 |
| Catalog Number | Size | Price | Quantity |
|---|---|---|---|
| A18-0006 | -- | -- |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
Ac-IETD-AFC is a fluorogenic substrate for caspase-8 and caspase-3. Caspase activity can be quantified by fluorescent detection of free AFC (7-amino-4-trifluoromethylcoumarin), which is excited at 400 nm and emits at 505 nm.
Chemical Information
Product Specification
Application
| Synonyms | N-Acetyl-Ile-Glu-Thr-Asp-7-amino-4-Trifluoromethylcoumarin; N-acetyl-L-isoleucyl-L-α-glutamyl-L-threonyl-N-[2-oxo-4-(trifluoromethyl)-2H-1-benzopyran-7-yl]-L-α-asparagine |
| Purity | ≥95% |
| Canonical SMILES | CCC(C)C(C(=O)NC(CCC(=O)O)C(=O)NC(C(C)O)C(=O)NC(CC(=O)O)C(=O)NC1=CC2=C(C=C1)C(=CC(=O)O2)C(F)(F)F)NC(=O)C |
| InChI | InChI=1S/C31H38F3N5O12/c1-5-13(2)25(35-15(4)41)29(49)37-19(8-9-22(42)43)27(47)39-26(14(3)40)30(50)38-20(12-23(44)45)28(48)36-16-6-7-17-18(31(32,33)34)11-24(46)51-21(17)10-16/h6-7,10-11,13-14,19-20,25-26,40H,5,8-9,12H2,1-4H3,(H,35,41)(H,36,48)(H,37,49)(H,38,50)(H,39,47)(H,42,43)(H,44,45)/t13-,14+,19-,20-,25-,26-/m0/s1 |
| InChIKey | CGUOWXRXLQXOKO-BYLHTXFJSA-N |
| Appearance | Solid Powder |
| Excitation | 400 nm |
| Emission | 505 nm |
| Storage | Store at -20°C |
Ac-IETD-AFC, a synthetic peptide substrate, plays a pivotal role in apoptosis research and enzymatic assays. Here are key applications of Ac-IETD-AFC:
Caspase-8 Activity Assays: Widely utilized for assessing caspase-8 activity, Ac-IETD-AFC enables the measurement of enzyme levels in cell lysates and tissue extracts. Upon cleaving Ac-IETD-AFC, caspase-8 releases AFC, a fluorescent compound that can be quantitatively analyzed. This method allows researchers to evaluate apoptotic responses across diverse experimental conditions, shedding light on cellular mechanisms in a dynamic fashion.
Drug Screening: Within the realm of pharmaceutical research, Ac-IETD-AFC serves as a valuable tool for screening potential therapeutic compounds capable of modulating caspase-8 activity. By gauging the impact of these compounds on caspase-8 inhibition or activation, scientists can pinpoint novel drugs with the potential to either impede or enhance apoptosis. This application holds particular significance in the realm of cancer chemotherapy, driving forward the quest for innovative treatment options.
Toxicology Studies: Within the sphere of toxicology research, Ac-IETD-AFC proves invaluable in interrogating the effects of environmental toxins and pharmacological agents on apoptosis pathways. By quantifying caspase-8 activity in treated cells, scientists can delineate how these substances modulate apoptotic processes. This analytical framework aids in evaluating the potential risks associated with exposure to diverse compounds, facilitating informed decision-making in toxicological studies.
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