Ac-IETD-AFC | CAS 211990-57-7

Catalog Number	A18-0006
Category	Fluorescent Enzyme Substrates
Molecular Formula	C31H38F3N5O12
Molecular Weight	729.7

Inquiry

* Please be kindly noted products are not for therapeutic use. We do not sell to patients.

Worldwide Delivery
Quality Assurance
24/7 Customer Service

Product Introduction

Ac-IETD-AFC is a fluorogenic probe that functions as a substrate for caspase-8 activity assays, featuring the amino acid sequence IETD linked to the 7-amino-4-trifluoromethylcoumarin (AFC) fluorophore. Upon enzymatic cleavage by caspase-8, the AFC moiety is liberated, resulting in a measurable increase in fluorescence emission, which can be detected using standard fluorescence spectroscopy techniques. This compound is used in apoptosis research to monitor caspase activity and facilitate the study of programmed cell death pathways.

Chemical Information
Product Specification
Application

Chemical Information

Synonyms	N-Acetyl-Ile-Glu-Thr-Asp-7-amino-4-Trifluoromethylcoumarin; N-acetyl-L-isoleucyl-L-α-glutamyl-L-threonyl-N-[2-oxo-4-(trifluoromethyl)-2H-1-benzopyran-7-yl]-L-α-asparagine
Purity	≥95%
SMILES	CCC(C)C(C(=O)NC(CCC(=O)O)C(=O)NC(C(C)O)C(=O)NC(CC(=O)O)C(=O)NC1=CC2=C(C=C1)C(=CC(=O)O2)C(F)(F)F)NC(=O)C
InChI	InChI=1S/C31H38F3N5O12/c1-5-13(2)25(35-15(4)41)29(49)37-19(8-9-22(42)43)27(47)39-26(14(3)40)30(50)38-20(12-23(44)45)28(48)36-16-6-7-17-18(31(32,33)34)11-24(46)51-21(17)10-16/h6-7,10-11,13-14,19-20,25-26,40H,5,8-9,12H2,1-4H3,(H,35,41)(H,36,48)(H,37,49)(H,38,50)(H,39,47)(H,42,43)(H,44,45)/t13-,14+,19-,20-,25-,26-/m0/s1
InChIKey	CGUOWXRXLQXOKO-BYLHTXFJSA-N
Appearance	Solid Powder