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Epoxy Fluor 7 | CAS 863223-43-2
| Catalog Number | A18-0064 |
| Category | Fluorescent Enzyme Substrates |
| Molecular Formula | C23H19NO5 |
| Molecular Weight | 389.4 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
Epoxy fluor 7 is a sensitive fluorescent substrate for soluble epoxide hydrolase (sEH) that can be used for human and mouse enzymes. Epoxy fluor 7 is hydrolyzed to yield fluorescence used for monitoring the activity of sEH.
Chemical Information
Product Specification
Application
| Synonyms | Cyano(6-methoxy-2-napthalenyl)methyl[(2,3)-3-phenyloxiranyl]methyl ester, carbonic acid |
| Purity | ≥98% |
| Canonical SMILES | COC1=CC2=C(C=C1)C=C(C=C2)C(C#N)OC(=O)OCC3C(O3)C4=CC=CC=C4 |
| InChI | InChI=1S/C23H19NO5/c1-26-19-10-9-16-11-18(8-7-17(16)12-19)20(13-24)29-23(25)27-14-21-22(28-21)15-5-3-2-4-6-15/h2-12,20-22H,14H2,1H3 |
| InChIKey | HDHFVICVWRKCHQ-UHFFFAOYSA-N |
| Appearance | Solid Powder |
| Excitation | 330 nm |
| Emission | 465 nm |
| Storage | Store at -20°C |
Epoxy Fluor 7, a versatile fluorescent compound, is extensively utilized in bioscience for diverse imaging and labeling tasks. Here are four key applications of Epoxy Fluor 7:
Fluorescence Microscopy: Playing a pivotal role in fluorescence microscopy, Epoxy Fluor 7 serves to stain and visualize cellular structures and proteins in biological samples with exceptional detail. Its intense fluorescence and remarkable photostability make it the go-to choice for intricate imaging tasks. This application empowers researchers to explore cell morphology, pinpoint specific proteins, and delve into cellular interactions with unparalleled precision.
Flow Cytometry: In the realm of flow cytometry, Epoxy Fluor 7 emerges as a fluorescent dye of choice for cell labeling, enabling the analysis of cell populations based on distinct markers. By tagging cells with Epoxy Fluor 7-conjugated antibodies, scientists can effectively measure and segregate cells exhibiting specific characteristics. This technique is indispensable, particularly in fields like immunology, aiding in unraveling immune responses and deciphering cellular functionalities.
Protein Labeling: Crucial for protein analysis, Epoxy Fluor 7 can be intricately linked to proteins, facilitating their detection and examination across various assays. This application plays a vital role in assays like enzyme-linked immunosorbent assays (ELISAs) and western blotting methodologies for the precise identification and quantification of target proteins. Through the labeling of proteins using Epoxy Fluor 7, researchers can glean valuable insights into protein expression, localization, and intricate interaction networks.
Live Cell Imaging: Revolutionizing live-cell imaging, Epoxy Fluor 7 is instrumental in tracking dynamic cellular processes in real-time, such as cell migration, division, and apoptosis. Its minimal toxicity and brilliant fluorescence characteristics enable prolonged observation of living cells without jeopardizing their viability. This application offers a wealth of information on cellular responses and processes under both physiological and experimental conditions.
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