Dnp-PYAYWMR | CAS 135662-06-5

Catalog Number	A18-0045
Category	Fluorescent Enzyme Substrates
Molecular Formula	C54H65N13O14S
Molecular Weight	1152.2

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Product Introduction

A Dnp-tagged peptide for studying proteolytic cleavage and enzyme kinetics. It shows rapid fluorescence recovery upon substrate hydrolysis.

Chemical Information
Product Specification
Application

Synonyms	Dnp-PYAYWMR (trifluoroacetate salt);Matrix Metalloproteinase-8 Fluorogenic Substrate I; MMP-8 Fluorogenic Substrate I; 1-(2,4-dinitrophenyl)-L-prolyl-L-tyrosyl-L-alanyl-L-tyrosyl-L-tryptophyl-L-methionyl-L-arginine
Purity	≥95%

Storage

Store at -20°C

Dnp-PYAYWMR is a fluorogenic heptapeptide substrate specifically designed to quantify matrix metalloproteinase-8 (MMP-8) activity. In its intact state, the N-terminal dinitrophenol (DNP) group quenches the fluorescence of the tryptophan residue within the peptide sequence. Upon enzymatic cleavage by MMP-8, the DNP group is removed, leading to the restoration of tryptophan fluorescence. This increase in fluorescence intensity is directly proportional to the level of MMP-8 activity, making Dnp-PYAYWMR a valuable tool for biochemical assays.

One key application of Dnp-PYAYWMR is in the field of medical research where it is utilized to study the role of MMP-8 in various diseases. MMP-8 is implicated in numerous pathological processes, including inflammation and tissue degradation. By using this substrate, researchers can measure the enzyme’s activity in different tissue samples, facilitating a better understanding of its involvement in disease progression and providing insights that could lead to the development of targeted therapies.

Another important usage of Dnp-PYAYWMR is in pharmacological testing. This peptide substrate is incorporated into high-throughput screening assays to evaluate the efficacy of potential MMP-8 inhibitors. By determining the level of enzyme activity in the presence of candidate drugs, researchers can identify compounds that effectively inhibit MMP-8, which is pivotal for developing new treatments for conditions characterized by excessive collagen breakdown and tissue remodeling, such as rheumatoid arthritis and certain cancers.

Dnp-PYAYWMR is also employed in biochemical research focused on enzyme kinetics. Researchers utilize this substrate to study the catalytic mechanisms of MMP-8, gathering data on enzyme-substrate interactions, reaction speeds, and the influence of various factors on enzyme function. Such detailed kinetic studies are crucial for comprehensively understanding how MMP-8 operates under physiological conditions, which can inform both basic research and therapeutic applications.

Finally, Dnp-PYAYWMR serves a crucial role in evaluating the efficacy of genetic or therapeutic interventions designed to modulate MMP-8 activity. For instance, in experiments where MMP-8 expression is knocked down or altered using genetic engineering techniques, this substrate allows scientists to accurately quantify changes in enzyme activity. This capability is essential for validating the success of interventions aimed at controlling MMP-8 levels, thereby expanding our capacity to manage diseases where this enzyme plays a critical role.