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Dibenzylfluorescein | 97744-44-0
| Catalog Number | A18-0002 |
| Category | Fluorescent Enzyme Substrates |
| Molecular Formula | C34H24O5 |
| Molecular Weight | 512.6 |
| Catalog Number | Size | Price | Quantity |
|---|---|---|---|
| A18-0002 | -- | $-- |
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Product Introduction
Dibenzylfluorescein is a fluorogenic probe that acts as a substrate for specific CYP3A4, CYP2C8, CYP2C9, CYP2C19, and aromatase (CYP19). Dibenzylfluorescein produces fluorescein benzyl ether when dealkylated by these CYP isoforms, which is further hydrolyzed to fluorescein by the addition of base (typically 2 M NaOH). It is used for detecting changes in catalytic activity by medication or the onset of disease.
Chemical Information |
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| Synonyms | 2-[3-oxo-6-(phenylmethoxy)-3H-xanthen-9-yl]-benzoic acid, phenylmethyl ester; DBF; O-Benzylfluorescein benzyl ester; Benzyl 2-[6-(benzyloxy)-3-oxo-3H-xanthen-9-yl]benzoate |
| Purity | ≥98% |
| Canonical SMILES | C1=CC=C(C=C1)COC2=CC3=C(C=C2)C(=C4C=CC(=O)C=C4O3)C5=CC=CC=C5C(=O)OCC6=CC=CC=C6 |
| InChI | InChI=1S/C34H24O5/c35-25-15-17-29-31(19-25)39-32-20-26(37-21-23-9-3-1-4-10-23)16-18-30(32)33(29)27-13-7-8-14-28(27)34(36)38-22-24-11-5-2-6-12-24/h1-20H,21-22H2 |
| InChI Key | YZJGKSLPSGPFEV-UHFFFAOYSA-N |
| Appearance | Orange Solid |
| Melting Point | >179°C (dec.) |
- Product Specification
- Application
| Excitation | 485 nm |
| Emission | 538 nm |
| Storage | Store at -20°C |
Dibenzylfluorescein, a fluorogenic substrate utilized in diverse biochemical and diagnostic settings, possesses a range of key applications. Here are four crucial applications of dibenzylfluorescein:
Enzyme Activity Assays: Widely adopted in enzyme assays, dibenzylfluorescein serves as a pivotal tool for measuring the activity of specific enzymes, such as cytochrome P450. Through metabolism by these enzymes, dibenzylfluorescein emits a fluorescent signal that can be precisely quantified. This application plays a critical role in deciphering enzyme functionality, screening potential drugs, and exploring intricate metabolic pathways.
Drug Metabolism Studies: In the realms of pharmacokinetics and drug discovery, dibenzylfluorescein proves invaluable for assessing the metabolic stability of novel drug candidates. By utilizing this substrate in liver microsomes or recombinant enzyme systems, researchers can ascertain the speed and efficiency of drug metabolism. This information is paramount for refining drug candidates and forecasting their behavior within the human body.
High-Throughput Screening: Dibenzylfluorescein finds extensive application in high-throughput screening assays aimed at identifying enzyme inhibitors or activators. Its fluorescent characteristics render it ideal for deployment in automated screening platforms, facilitating the simultaneous assessment of thousands of compounds. This expedites the drug discovery process and aids in the recognition of potential therapeutic agents.
Toxicology Studies: Within the domain of toxicology, dibenzylfluorescein plays a crucial role in evaluating the potential toxicity of chemical compounds by examining their effects on enzyme activity. Researchers can track changes in fluorescence to determine if a compound hinders or enhances specific enzymes. This analytical approach is essential for comprehending the toxicological profiles of substances and guaranteeing their safety in pharmaceutical and environmental contexts.
Applications of Fluorescent Probes & Dyes
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