Dibenzylfluorescein | CAS 97744-44-0

Catalog Number	A18-0002
Category	Fluorescent Enzyme Substrates
Molecular Formula	C34H24O5
Molecular Weight	512.6

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Product Introduction

Dibenzylfluorescein is a fluorogenic substrate for esterase and lipase assays. It produces bright green fluorescence for enzyme activity measurement.

Chemical Information
Product Specification
Application

Synonyms	2-[3-oxo-6-(phenylmethoxy)-3H-xanthen-9-yl]-benzoic acid, phenylmethyl ester; DBF; O-Benzylfluorescein benzyl ester; Benzyl 2-[6-(benzyloxy)-3-oxo-3H-xanthen-9-yl]benzoate
Purity	≥98%
Canonical SMILES	C1=CC=C(C=C1)COC2=CC3=C(C=C2)C(=C4C=CC(=O)C=C4O3)C5=CC=CC=C5C(=O)OCC6=CC=CC=C6
InChI	InChI=1S/C34H24O5/c35-25-15-17-29-31(19-25)39-32-20-26(37-21-23-9-3-1-4-10-23)16-18-30(32)33(29)27-13-7-8-14-28(27)34(36)38-22-24-11-5-2-6-12-24/h1-20H,21-22H2
InChIKey	YZJGKSLPSGPFEV-UHFFFAOYSA-N
Appearance	Orange Solid
Melting Point	>179°C (dec.)

Excitation	485 nm
Emission	538 nm
Storage	Store at -20°C

Dibenzylfluorescein, a fluorogenic substrate utilized in diverse biochemical and diagnostic settings, possesses a range of key applications. Here are four crucial applications of dibenzylfluorescein:

Enzyme Activity Assays: Widely adopted in enzyme assays, dibenzylfluorescein serves as a pivotal tool for measuring the activity of specific enzymes, such as cytochrome P450. Through metabolism by these enzymes, dibenzylfluorescein emits a fluorescent signal that can be precisely quantified. This application plays a critical role in deciphering enzyme functionality, screening potential drugs, and exploring intricate metabolic pathways.

Drug Metabolism Studies: In the realms of pharmacokinetics and drug discovery, dibenzylfluorescein proves invaluable for assessing the metabolic stability of novel drug candidates. By utilizing this substrate in liver microsomes or recombinant enzyme systems, researchers can ascertain the speed and efficiency of drug metabolism. This information is paramount for refining drug candidates and forecasting their behavior within the human body.

High-Throughput Screening: Dibenzylfluorescein finds extensive application in high-throughput screening assays aimed at identifying enzyme inhibitors or activators. Its fluorescent characteristics render it ideal for deployment in automated screening platforms, facilitating the simultaneous assessment of thousands of compounds. This expedites the drug discovery process and aids in the recognition of potential therapeutic agents.

Toxicology Studies: Within the domain of toxicology, dibenzylfluorescein plays a crucial role in evaluating the potential toxicity of chemical compounds by examining their effects on enzyme activity. Researchers can track changes in fluorescence to determine if a compound hinders or enhances specific enzymes. This analytical approach is essential for comprehending the toxicological profiles of substances and guaranteeing their safety in pharmaceutical and environmental contexts.