Cyanine5 alkyne | CAS 1223357-57-0

Cyanine5 alkyne is a cyanine-based fluorescent labeling reagent bearing an alkyne handle for copper-catalyzed azide-alkyne cycloaddition (CuAAC) click chemistry. This dye is designed to be incorporated into biomolecule conjugates, imaging probes, and polymer or surface constructs where far-red fluorescence is advantageous, enabling visualization and traceable tracking in fluorescence microscopy and related analytical workflows.

1. Click Bioconjugation Labeling

Cyanine5 alkyne is used by chemical biology and glycobiology groups to generate fluorescently labeled biomolecules through CuAAC with azide-bearing partners. Researchers commonly attach it to proteins, peptides, and other biomolecular scaffolds that have been functionalized with azide groups, creating stable fluorescent conjugates for downstream imaging, binding studies, and reagent development. The cyanine dye's far-red emission supports multiplex-friendly experimental designs where spectral separation from visible fluorophores is required.

2. Fluorescence Microscopy Tracing

Cyanine5 alkyne is applied in fluorescence microscopy workflows where click-labeled targets need to be tracked with high contrast in complex samples. Teams in cell imaging and materials biology use it to fluorescently tag biomolecule pathways, extracellular components, or engineered constructs by clicking the dye onto azide-functionalized probes or surfaces. The resulting conjugates are frequently used for localization studies, colocalization experiments, and time-course imaging in microscopy platforms compatible with far-red detection.

3. Labeled Biomaterial And Surface Engineering

Cyanine5 alkyne supports biomaterials and surface modification programs that require fluorescent readouts of functionalized materials. Researchers incorporate the dye into hydrogels, coatings, and nanoparticle or surface architectures by reacting it with azide-functional groups present on the material scaffold. This enables mapping of surface coverage, monitoring of conjugation uniformity, and visualization of material-associated biomolecule binding events using standard fluorescence imaging instrumentation.

4. Fluorescent Probe Construction

Cyanine5 alkyne is leveraged in fluorescent probe development when a click-attachable cyanine fluorophore is needed for modular assembly. In probe engineering workflows, scientists use the alkyne handle to build conjugates where the dye is introduced late in the design cycle, improving flexibility for generating libraries of imaging reagents. The far-red cyanine signal is particularly useful for constructing imaging probes intended for fluorescence-based readouts in assay development and molecular imaging studies.