Cell proliferation tracer Fluorescent Probes
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Cell proliferation tracer fluorescent probes are widely used in the detection of cell proliferation and fluorescent tracking of cells in recent years. The excitation wavelength can be selected in 488 nm, and the emission wavelength are 518 nm. They can be prepared with anhydrous DMSO, usually at a concentration of 2-10 mM. The prepared solution should be used up on the day and not be stored for a long time.
Characteristics of cell proliferation tracer fluorescent probes
Cell proliferation tracer fluorescent probes can penetrate the cell membrane, and can be decomposed by intracellular esterase. Decomposition product can spontaneously and irreversibly combine with Lysine residues or other amino groups of intracellular proteins. Labeling cells with cell proliferation tracker fluorescent probes takes only 5-15 minutes. The fluorescence of the labeled cells by the probe was very uniform, and the fluorescence distribution of the progeny cells after division was also uniform. The fluorescence of non-dividing cells labeled with the probe are very stable, and the labeling time are for several months. The labeled cells by the probe did not stain adjacent cells either in vitro or in vivo. That are, they will not be transferred from one cell to adjacent cells after labeling.
Application of cell proliferation tracer fluorescent probes
Cell proliferation that divides up to 8 or more times can be detected using cell proliferation tracer fluorescent probes. Currently, these probes are most commonly used to detect the proliferation of lymphocytes. They can also be used for the detection of the proliferation of other cells such as fibroblasts and NK cells. They can even be used for the detection of bacterial proliferation.