CellHunt Far Red DDAO-SE

CellHunt Far Red DDAO-SE is a far-red fluorescent labeling reagent based on DDAO (5-(4,6-dichlorotriazinyl)aminofluorescein) chemistry, supplied as an SE (succinimidyl ester) activated dye for covalent attachment to primary amines. In typical workflows, it is used to generate bright far-red conjugates for fluorescence microscopy and quantitative imaging, taking advantage of its long-wavelength emission to reduce spectral overlap with common green/orange channels.

1. Fluorescent Protein Labeling

CellHunt Far Red DDAO-SE is widely used by cell biology and protein chemistry groups to fluorescently label antibodies, enzymes, and other amine-containing proteins for imaging and assay development. After conjugation to lysine residues or N-termini, the far-red emission supports multicolor experiments where green dyes may interfere, enabling tracking of labeled biomolecules in fixed-cell studies, binding assays, and fluorescence readouts that benefit from reduced background from autofluorescence.

2. Flow Cytometry Staining

CellHunt Far Red DDAO-SE is used in flow cytometry workflows to prepare far-red fluorescent reagents for staining and analysis of amine-bearing targets, including labeled affinity reagents and secondary detection constructs. Researchers often incorporate the dye-labeled conjugates into antibody-based staining panels to separate signals from standard fluorophores, supporting quantitative comparisons across experimental conditions while maintaining compatibility with common far-red detection channels.

3. Fluorescence Microscopy Tracing

CellHunt Far Red DDAO-SE supports fluorescence microscopy tracing of labeled biomolecules and probes in cellular imaging experiments where long-wavelength detection improves contrast. In practice, it is used to visualize conjugate localization patterns, follow uptake or binding of labeled reagents, and generate far-red imaging reagents for time-course studies in fixed samples, with the SE functionality enabling robust covalent attachment to amine-containing components of the imaging construct.

4. Biomaterial Surface Functionalization

CellHunt Far Red DDAO-SE is applied in biomaterials and materials chemistry to introduce far-red fluorescence onto amine-functional surfaces and polymers for imaging-based characterization. Teams use the dye to create fluorescently tagged coatings or labeled materials that can be visualized during surface adsorption studies, coating uniformity checks, and microscopy-based assessment of material interactions with biological samples, leveraging the succinimidyl ester for stable coupling to surface-exposed primary amines.