Lucifer Yellow CH Dipotassium Salt | 71206-95-6

Lucifer Yellow CH Dipotassium Salt, a highly fluorescent dye extensively utilized in scientific research, serves a multitude of purposes. Here are four key applications of this dye:

Cellular Dyeing and Tracing: Lucifer Yellow emerges as a pivotal tool for cell staining and tracing owing to its intense fluorescence and remarkable water solubility. Scientists harness its brilliance to label neurons, glial cells, and diverse cell types, enabling visualization under a fluorescence microscope.

Intracellular Injection: This dye finds frequent application through microinjection into individual cells, facilitating the study of intracellular processes and cell-to-cell connectivity. By monitoring the diffusion of Lucifer Yellow, researchers unveil neuronal circuits, unraveling the intricacies of cellular communication dynamics. Particularly vital in neurobiology, this technique aids in mapping brain structures and pathways with precision.

Gap Junction Analysis: In dissecting gap junction communication among cells, Lucifer Yellow plays a pivotal role. Its petite molecular size grants it passage through gap junctions, offering a valuable tool for evaluating connectivity and functionality of these cellular conduits. Researchers gain insights into cellular coupling efficiency by tracking the dye’s diffusion, shedding light on cellular interactions.

Live Cell Imaging: Delving into live cell analysis, Lucifer Yellow facilitates real-time monitoring of cellular processes with unparalleled precision. Its low toxicity and photostability render it ideal for extended imaging endeavors. Researchers harness its capabilities to explore dynamic cellular activities like endocytosis, exocytosis, and intracellular transport pathways, enhancing our understanding of intricate cellular dynamics.