Resazurin sodium salt

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Resazurin sodium salt

Resazurin sodium salt | 62758-13-8

Catalog Number A16-0029
Category Cell proliferation tracer Fluorescent Probes
Molecular Formula C12H6NNaO4
Molecular Weight 251.2
Catalog Number Size Price Quantity
A16-0029 -- $--

Product Introduction

Resazurin is a blue non-fluorescent dye that is used as an oxidation-reduction indicator in cell viability assays. Resazurin generates red fluorescence when reduced to resorufin.

Chemical Information

Related CAS 550-82-3 (free base)
Synonyms 7-hydroxy-3H-phenoxazin-3-one-10-oxide monosodium salt
Canonical SMILES C1=CC2=C(C=C1[O-])OC3=CC(=O)C=CC3=[N+]2[O-].[Na+]
InChI InChI=1S/C12H7NO4.Na/c14-7-1-3-9-11(5-7)17-12-6-8(15)2-4-10(12)13(9)16;/h1-6,14H;/q;+1/p-1
InChI Key IVGPGQSSDLDOLH-UHFFFAOYSA-M
Appearance Solid Powder
  • Product Specification
  • Application
Excitation 530-540 nm
Emission 585-595 nm
Storage Store at -20°C

Resazurin sodium salt, a versatile redox indicator, finds wide-ranging applications in scientific research. Here are four key applications of Resazurin sodium salt:

Cell Viability Assays: Widely employed in cell viability and cytotoxicity assays, Resazurin sodium salt stands out for its unique ability to gauge metabolic activity in cells. Viable cells catalyze the reduction of resazurin to resorufin, emitting a fluorescent signal. This characteristic enables researchers to quantitatively assess cell proliferation, viability, and the cytotoxic effects of compounds in a precise and dependable manner.

Microbial Testing: A pivotal tool in microbiology, resazurin sodium salt functions as a reliable indicator for bacterial and yeast proliferation. When introduced into culture media, the reduction of resazurin to resorufin serves as a telltale sign of microbial growth. This technique is commonly utilized in antibiotic susceptibility tests and the quality control of microbiological processes.

Environmental Toxicology: In the realm of environmental studies, resazurin sodium salt plays a crucial role in evaluating the toxicity of pollutants on aquatic organisms. By subjecting cultured cells or microorganisms to environmental samples containing contaminants, the reduction of resazurin offers valuable insights into the detrimental effects of pollutants. This aids in the continuous monitoring of environmental well-being and the assessment of pollutant impacts.

Biofilm Formation Studies: Researchers turn to resazurin sodium salt to delve into the intricacies of biofilm formation by diverse microbial species. By examining the metabolic activity within biofilms, this compound serves as a key tool in unraveling the dynamics of biofilm structures. This application holds particular significance in medical research, contributing to the development of effective strategies for managing biofilm-related infections.

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