Green CMFDA | CAS 136832-63-8

Catalog Number	A16-0024
Category	Cell Proliferation Tracer Fluorescent Probes
Molecular Formula	C25H17ClO7
Molecular Weight	464.9

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Product Introduction

Green CMFDA is a thiol reactive and cell-permeable green fluorescent probe that freely enters cell membranes. Green CMFDA shows stability and nontoxicity in working concentration and is well retained in cells at physiological pH, therefore it has been an ideal cell tracking probe.

Chemical Information
Product Specification
Application

Synonyms	5-Chloromethylfluorescein diacetate; 3',6'-bis(acetyloxy)-5-(chloromethyl)-spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
Purity	≥95%
Canonical SMILES	CC(=O)OC1=CC2=C(C=C1)C3(C4=C(C=C(C=C4)CCl)C(=O)O3)C5=C(O2)C=C(C=C5)OC(=O)C
InChI	InChI=1S/C25H17ClO7/c1-13(27)30-16-4-7-20-22(10-16)32-23-11-17(31-14(2)28)5-8-21(23)25(20)19-6-3-15(12-26)9-18(19)24(29)33-25/h3-11H,12H2,1-2H3
InChIKey	IPJDHSYCSQAODE-UHFFFAOYSA-N
Appearance	Solid Powder

Excitation	492 nm
Emission	517 nm
Storage	Store at -20°C

Green CMFDA, a cell-permeant fluorescent dye, finds versatile applications in various biological contexts. Here are four key applications of Green CMFDA:

Cell Viability Assays: Within the realm of cell viability assays, Green CMFDA emerges as a pivotal tool for assessing the health and survival of cultured cells. This fluorescent dye effortlessly penetrates live cells, undergoing conversion into a fluorescent product that signals active metabolism. Researchers can thus gauge the impact of diverse treatments or environmental stimuli on cell viability, unraveling the intricate dynamics of cellular survival mechanisms.

Cell Tracking: Delving into cell tracking endeavors, Green CMFDA showcases its prowess by labeling live cells for prolonged observation of their migratory patterns and spatial distribution. With its enduring fluorescence, cells tagged with Green CMFDA can be meticulously monitored over extended periods, both in vivo and in vitro. This application proves invaluable in diverse fields such as migration studies, immunology, and developmental biology, shedding light on the nuanced behaviors of living cells.

Fluorescence Microscopy: Stepping into the domain of fluorescence microscopy, Green CMFDA takes center stage as a go-to option for real-time visualization and analysis of live cells. Its vibrant green fluorescence lends itself perfectly to the observation of cellular morphology, behavior, and interactions under the magnifying lens of a microscope. This indispensable technique finds applications in diverse disciplines including cell biology, neuroscience, and cancer research, offering insights into the dynamic world of cellular activities.

Flow Cytometry: In the intricate landscape of flow cytometry, Green CMFDA assumes a crucial role as a fluorescent dye for interrogating cell populations based on their viability and metabolic activity. When combined with other fluorescent markers, Green CMFDA aids in the differentiation of distinct cell types and the identification of subpopulations within a sample. This application holds significance in tasks such as immunophenotyping, cell sorting, and diagnostics, enabling researchers to unravel the intricate tapestry of cellular heterogeneity and functionality.