AMPPD

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AMPPD

AMPPD | 122341-56-4

Catalog Number A18-0070
Category Fluorescent Enzyme Substrates
Molecular Formula C18H23O7P
Molecular Weight 382.34
Catalog Number Size Price Quantity
A18-0070 -- $--

Product Introduction

AMPPD is a sensitive fluorogenic substrate for alkaline phosphatase. It is mainly used for immunodetection, such as tumor markers, infectious diseases, endocrine function, hormones and other aspects of the diagnosis.

Chemical Information

Related CAS 124951-96-8 (disodium salt)
Synonyms 3-[2-spiroadamatane]-4-methoxy-4-[3-phosphoryloxy]-phenyl-1,2-dioxetane; 3-(4'-Methoxyspiro[adamantane-2,3'-[1,2]dioxetan]-4'-yl)phenyl dihydrogen phosphate; 3-(2'-Spiroadamantane)-4-methoxy-4-(3''-phosphoryloxy)phenyl-1,2-dioxetane; 4-Methoxy-4-(3-phosphatephenyl)spiro(1,2-dioxetane)-3,2'-adamantane; 3-(4-Methoxyspiro(1,2-dioxetane-3,2'-tricyclo(3.3.1.1(3,7))decan)-4-yl)phenyl phosphate; Lumigen PPD
Purity ≥98% by HPLC
IUPAC Name [3-(3'-methoxyspiro[adamantane-2,4'-dioxetane]-3'-yl)phenyl] dihydrogen phosphate
Canonical SMILES COC1(C2(C3CC4CC(C3)CC2C4)OO1)C5=CC(=CC=C5)OP(=O)(O)O
InChI InChI=1S/C18H23O7P/c1-22-18(13-3-2-4-16(10-13)23-26(19,20)21)17(24-25-18)14-6-11-5-12(8-14)9-15(17)7-11/h2-4,10-12,14-15H,5-9H2,1H3,(H2,19,20,21)
InChI Key XYIPYISRNJUPBA-UHFFFAOYSA-N
Solubility Soluble in Water
Density 1.47±0.1 g/cm3 (Predicted)
Appearance White Powder
Boiling Point 548.6±60.0°C (Predicted)
  • Product Specification
  • Application
Storage -20°C

AMPPD, known as 3-(2’-spiroadamantane)-4-methoxy-4-(3’’-phosphoryloxy)phenyl-1,2-dioxetane, is a widely used chemiluminescent substrate in various bioscience applications. Here are four key applications of AMPPD:

Molecular Diagnostics: In the realm of diagnostics, AMPPD plays a pivotal role in detecting specific nucleic acids and proteins with precision. In assays like ELISA, the enzyme alkaline phosphatase catalyzes the conversion of AMPPD into a luminescent product, enabling sensitive detection. This enhanced accuracy is instrumental in diagnostic tests for diseases ranging from cancer to infectious diseases.

Western Blotting: Utilized in Western blotting, AMPPD acts as a substrate for visualizing alkaline phosphatase-labeled antibodies. The ensuing chemiluminescent reaction produces a signal directly proportional to the target protein’s abundance in the sample. This method empowers researchers to visualize and quantify specific proteins with exceptional sensitivity and accuracy.

Bioluminescent Imaging: Emerging as a cornerstone in biological research, AMPPD is integral to bioluminescent imaging techniques that offer real-time insights into cellular activities. By leveraging genetically modified cells or organisms expressing alkaline phosphatase, researchers can track disease progression, gene expression, and therapeutic responses. This real-time monitoring is invaluable for unraveling the intricate dynamics of biological systems.

Environmental Monitoring: Expanding into environmental science, AMPPD-based assays are deployed for detecting contaminants like pathogens and pollutants in water and soil samples. The chemiluminescent signal produced by AMPPD allows for precise quantification of these substances with heightened sensitivity. These rapid and accurate detection methods bolster environmental safety and public health efforts.

Computed Properties

XLogP3 1.7
Hydrogen Bond Donor Count 2
Hydrogen Bond Acceptor Count 7
Rotatable Bond Count 4
Exact Mass 382.11814007 g/mol
Monoisotopic Mass 382.11814007 g/mol
Topological Polar Surface Area 94.4Ų
Heavy Atom Count 26
Formal Charge 0
Complexity 591
Isotope Atom Count 0
Defined Atom Stereocenter Count 0
Undefined Atom Stereocenter Count 1
Defined Bond Stereocenter Count 0
Undefined Bond Stereocenter Count 0
Covalently-Bonded Unit Count 1
Compound Is Canonicalized Yes

Patents

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CN-115073492-A An olanzapine derivative antigen, olanzapine antibody and application thereof 2022-08-22
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