AMPPD | 122341-56-4

AMPPD, known as 3-(2’-spiroadamantane)-4-methoxy-4-(3’’-phosphoryloxy)phenyl-1,2-dioxetane, is a widely used chemiluminescent substrate in various bioscience applications. Here are four key applications of AMPPD:

Molecular Diagnostics: In the realm of diagnostics, AMPPD plays a pivotal role in detecting specific nucleic acids and proteins with precision. In assays like ELISA, the enzyme alkaline phosphatase catalyzes the conversion of AMPPD into a luminescent product, enabling sensitive detection. This enhanced accuracy is instrumental in diagnostic tests for diseases ranging from cancer to infectious diseases.

Western Blotting: Utilized in Western blotting, AMPPD acts as a substrate for visualizing alkaline phosphatase-labeled antibodies. The ensuing chemiluminescent reaction produces a signal directly proportional to the target protein’s abundance in the sample. This method empowers researchers to visualize and quantify specific proteins with exceptional sensitivity and accuracy.

Bioluminescent Imaging: Emerging as a cornerstone in biological research, AMPPD is integral to bioluminescent imaging techniques that offer real-time insights into cellular activities. By leveraging genetically modified cells or organisms expressing alkaline phosphatase, researchers can track disease progression, gene expression, and therapeutic responses. This real-time monitoring is invaluable for unraveling the intricate dynamics of biological systems.

Environmental Monitoring: Expanding into environmental science, AMPPD-based assays are deployed for detecting contaminants like pathogens and pollutants in water and soil samples. The chemiluminescent signal produced by AMPPD allows for precise quantification of these substances with heightened sensitivity. These rapid and accurate detection methods bolster environmental safety and public health efforts.