Live Imaging of the Extracellular Matrix With a Glycan-Binding Fluorophore

June 9th, 2026 10:00 AM EDT

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Specific Fluorescent Stains
Fluorescent Enzyme Substrates
6,8-difluoro-4-methylumbelliferyl phosphate

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6,8-difluoro-4-methylumbelliferyl phosphate

6,8-difluoro-4-methylumbelliferyl phosphate | CAS 214491-43-7

Catalog Number	A18-0076
Category	Fluorescent Enzyme Substrates
Molecular Formula	C10H7F2O6P
Molecular Weight	292.13

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Product Introduction

6,8-Difluoro-4-methylumbelliferyl phosphate is a non-fluorescent substrate that becomes fluorescent upon enzymatic dephosphorylation, making it a useful tool for monitoring phosphatase activity in biochemical assays. This compound features a coumarin-based fluorophore, which, upon conversion to its free phenolic form, exhibits enhanced fluorescence emission, facilitating its application in fluorescence-based enzyme assays. Its ability to serve as a phosphatase substrate enables its incorporation into various experimental workflows aimed at studying enzyme kinetics and activity in cellular and biochemical research contexts.

Chemical Information
Application

Chemical Information

Synonyms	DiFMUP; (6,8-Difluoro-4-methyl-2-oxochromen-7-yl) dihydrogen phosphate
SMILES	CC1=CC(=O)OC2=C(C(=C(C=C12)F)OP(=O)(O)O)F
InChI	InChI=1S/C10H7F2O6P/c1-4-2-7(13)17-9-5(4)3-6(11)10(8(9)12)18-19(14,15)16/h2-3H,1H3,(H2,14,15,16)
InChIKey	DZANYXOTJVLAEE-UHFFFAOYSA-N
Appearance	Solid Powder

Application

6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP), a widely used fluorogenic substrate in bioscience research, has diverse applications. Here are four key applications of DiFMUP:

Enzyme Activity Assays: Serving as a substrate for phosphatase enzyme activity assays, DiFMUP undergoes dephosphorylation by phosphatases, resulting in the release of a fluorescent product for easy detection and quantification. This unique property makes DiFMUP an exceptional tool for measuring enzyme kinetics and screening potential inhibitors in drug discovery, offering insights into the intricate world of enzymatic reactions.

Cell Signaling Studies: In the realm of cell signaling pathways, DiFMUP finds its place by aiding in the quantification of phosphatase activity, particularly vital in understanding signal transduction processes under diverse conditions. By decoding how these enzymes function, researchers gain profound insights into cellular responses to various stimuli, unraveling potential therapeutic targets amidst the complex web of intracellular communication.

Cancer Research: Embracing the realm of cancer research, DiFMUP emerges as a valuable tool for studying phosphatase activity in tumor cells. With variations in phosphatase activity serving as potential indicators of oncogenic processes, DiFMUP-based assays offer a window into diagnosing and characterizing different cancer types.

High-Throughput Screening: Equipped for high-throughput screening (HTS) assays in pharmaceutical research, DiFMUP’s fluorogenic properties pave the way for rapid and sensitive detection of enzyme activity within vast compound libraries. This streamlined approach accelerates the identification of new drug candidates, propelling the drug development process forward with agility and precision, shaping the future of pharmaceutical innovation.