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5-(6)-CDCFDA | CAS 127770-45-0
| Catalog Number | A18-0072 |
| Category | Fluorescent Enzyme Substrates |
| Molecular Formula | C25H14Cl2O9 |
| Molecular Weight | 529.28 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
5-(6)-CDCFDA is a membrane-permeable substrate for esterase that can be hydrolyzed in cells to yield the fluorescent product. It can be used as an acidic pH sensor With a pKa of 4.8.
Chemical Information
Product Specification
Application
| Synonyms | 5(6)-Carboxy-2',7'-dichlorofluorescein Diacetate |
| Excitation | Non-Activated, Amine-Reactive (Free Acid) |
5-(6)-CDCFDA, a fluorescent dye commonly employed in diverse bioscience applications, offers a spectrum of uses in various research domains. Here are four key applications of 5-(6)-CDCFDA:
Cell Viability Assays: Integral to cell viability assessments, 5-(6)-CDCFDA serves as a crucial component in monitoring live cells’ status. Following uptake by viable cells, the dye undergoes conversion to a fluorescent derivative, enabling researchers to quantify cell viability through fluorescence microscopy or flow cytometry. This application plays a pivotal role in evaluating the efficacy of drugs and treatments in cell culture studies, shedding light on cellular responses and potential treatment outcomes.
Intracellular Enzyme Activity: Within the realm of intracellular enzyme activity measurements, 5-(6)-CDCFDA emerges as a key tool for evaluating esterase activities in live cells. Upon cellular entry, 5-(6)-CDCFDA undergoes hydrolysis by esterases, releasing a fluorescent compound. By monitoring the fluorescence intensity, researchers can gauge enzyme activity levels, providing insights into cellular health and metabolic processes. This method offers a window into cellular functions, aiding in the understanding of critical metabolic pathways and enzymatic responses.
Flow Cytometry: In the realm of flow cytometry investigations, 5-(6)-CDCFDA finds application in labeling and analyzing living cells based on fluorescence signals. The dye’s fluorescence properties enable the discrimination between live and dead cells, enabling detailed analysis of cell populations under varying experimental conditions. Widely utilized in immunology, cancer research, and other fields, this technique offers a nuanced perspective on cellular behavior and properties, facilitating comprehensive studies on cell dynamics and responses.
Reactive Oxygen Species (ROS) Detection: Beyond its conventional uses, 5-(6)-CDCFDA plays a pivotal role in detecting reactive oxygen species (ROS) within cellular environments. Upon oxidation by ROS, the dye emits fluorescence, enabling researchers to quantify oxidative stress levels across diverse experimental setups. This application holds critical importance in elucidating the impact of oxidative stress in various diseases and assessing the effectiveness of antioxidants in mitigating cellular damage. By providing insights into cellular oxidative balance, this technique contributes to advancing our understanding of disease mechanisms and therapeutic interventions.
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