N6-(6-Aminohexyl)-ATP-6-FAM Triethylammonium salt

Name: N6-(6-Aminohexyl)-ATP-6-FAM Triethylammonium salt
Brand: BOC Sciences
Rating: 5 (1 reviews)

Catalog Number	F04-0054
Category	Fluorescein FAM
Molecular Formula	C37H39N6O19P3 (free acid)
Molecular Weight	964.66 (free acid)

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Product Introduction

N6-(6-Aminohexyl)-ATP-6-FAM Triethylammonium salt is a fluorescent probe that integrates the ATP molecule with the 6-FAM fluorophore, a member of the fluorescein dye family known for its bright green fluorescence. This compound features an aminohexyl linker that facilitates conjugation to biomolecules, enabling its use in various biochemical labeling and imaging applications. The triethylammonium salt form enhances solubility, making it suitable for incorporation into assays that require specific nucleotide labeling and detection through fluorescence microscopy or flow cytometry.

Chemical Information
Product Specification
Application

Chemical Information

Purity	≥95% by HPLC
IUPAC Name	4-((6-((9-((2R,3R,4S,5R)-3,4-dihydroxy-5-(((hydroxy((hydroxy(phosphonooxy)phosphoryl)oxy)phosphoryl)oxy)methyl)tetrahydrofuran-2-yl)-9H-purin-6-yl)amino)hexyl)carbamoyl)-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid;triethylammonium salt
Solubility	Soluble in Water
Appearance	Yellow Liquid

Product Specification

Excitation	492 nm
Emission	517 nm
Storage	Store at -20°C

Application

N6-(6-Aminohexyl)-ATP-6-FAM Triethylammonium salt is a fluorescent ATP analog designed for tracking ATP-dependent processes and for building fluorescent nucleotide probes. The molecule combines an ATP core with a primary amine handle for conjugation and a 6-FAM fluorophore for excitation/emission readout in fluorescence microscopy and plate-based assays, enabling direct visualization of nucleotide utilization and ATP-binding workflows.

1. ATP-Analog Labeling Assays

N6-(6-Aminohexyl)-ATP-6-FAM Triethylammonium salt is used by enzymology and chemical biology groups to monitor ATP-dependent reactions where fluorescent nucleotide analogs provide a convenient readout. Researchers incorporate the reagent into fluorescence-based activity assays to follow substrate turnover, nucleotide incorporation, or ATP utilization in purified enzyme systems and cell lysate formats, leveraging the FAM signal for kinetic measurements and endpoint comparisons.

2. Fluorescent Nucleotide Probe Building

N6-(6-Aminohexyl)-ATP-6-FAM Triethylammonium salt supports probe construction workflows that require a reactive amine for downstream coupling. The 6-aminohexyl functionality enables conjugation to targeting ligands, affinity handles, or immobilization supports, allowing teams to generate fluorescent nucleotide probes for studying nucleotide interactions, ATP-binding surfaces, and ATP-associated biomolecular assemblies in imaging and analytical experiments.

3. Bioconjugation For Imaging Studies

N6-(6-Aminohexyl)-ATP-6-FAM Triethylammonium salt is applied in molecular imaging reagent development when fluorescent nucleotide analogs are needed to visualize ATP-related binding in biochemical preparations. With its amine handle, researchers can prepare conjugates for microscopy workflows where fluorescent labeling of ATP-associated components is required, including studies of nucleotide engagement on biomaterials, immobilized proteins, and engineered assay surfaces.

4. Surface Immobilized ATP Tracking

N6-(6-Aminohexyl)-ATP-6-FAM Triethylammonium salt is used to functionalize surfaces and biomaterial interfaces for fluorescence readouts of ATP interaction. Materials scientists and assay developers couple the amine-bearing nucleotide analog to solid supports to create ATP-responsive fluorescent coatings or immobilized probe platforms, enabling washing-based experiments and spatially resolved fluorescence measurements to assess nucleotide association on engineered materials.