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HEX azide, 6-isomer | CAS 1450752-91-6
| Catalog Number | F04-0052 |
| Category | Fluorescein FAM |
| Molecular Formula | C24H12N4Cl6O6 |
| Molecular Weight | 665.09 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
Hexachlorofluorescein (HEX) is a hexachlorinated derivate of the fluorescent dye fluorescein. HEX-labeled oligonucleotides are used in PCR and the HEX channel is widely used in multiplex qPCR.,Oligonucleotides with a HEX label can be easily generated using HEX azide and alkyne-containing oligonucleotide via azide-alkyne cycloaddition.,HEX exhibits an excitation peak at 533 nm and an emission peak at 549 in green-yellow area, and it can serve as a substitute for JOE, BODIPY™ 530/550, VIC™ because of the similar spectral characteristics.
Chemical Information
Product Specification
Computed Properties
| Synonyms | 6-Hexachloro-Fluorescein-Azide |
| Purity | NMR 1H, HPLC-MS (95%) |
| IUPAC Name | N-(3-azidopropyl)-2',4,4',5',7,7'-hexachloro-3',6'-dihydroxy-1-oxospiro[2-benzofuran-3,9'-xanthene]-5-carboxamide |
| Canonical SMILES | C1=C(C(=C2C(=C1Cl)C(=O)OC23C4=CC(=C(C(=C4OC5=C(C(=C(C=C35)Cl)O)Cl)Cl)O)Cl)Cl)C(=O)NCCCN=[N+]=[N-] |
| InChI | InChI=1S/C24H12Cl6N4O6/c25-10-4-7(22(37)32-2-1-3-33-34-31)15(28)14-13(10)23(38)40-24(14)8-5-11(26)18(35)16(29)20(8)39-21-9(24)6-12(27)19(36)17(21)30/h4-6,35-36H,1-3H2,(H,32,37) |
| InChIKey | HGKPJGGICCDTJN-UHFFFAOYSA-N |
| Solubility | DMSO, DMF |
| Appearance | Orange solid |
| CF260 | 0.30 |
| CF280 | 0.13 |
| Fluorescence Quantum Yield | 0.57 |
| Mass Spec M+ Increment | 661.9 |
| Excitation | 533 |
| Emission | 549 |
| Storage | Storage: 24 months after receival at -20 °C in the Dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| XLogP3 | 7.7 |
| Hydrogen Bond Donor Count | 3 |
| Hydrogen Bond Acceptor Count | 8 |
| Rotatable Bond Count | 5 |
| Exact Mass | 663.885850 g/mol |
| Monoisotopic Mass | 661.888800 g/mol |
| Topological Polar Surface Area | 119Ų |
| Heavy Atom Count | 40 |
| Formal Charge | 0 |
| Complexity | 1020 |
| Isotope Atom Count | 0 |
| Defined Atom Stereocenter Count | 0 |
| Undefined Atom Stereocenter Count | 0 |
| Defined Bond Stereocenter Count | 0 |
| Undefined Bond Stereocenter Count | 0 |
| Covalently-Bonded Unit Count | 1 |
| Compound Is Canonicalized | Yes |
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