Fluorescein II CEP | CAS 1027512-13-5

Name: Fluorescein II CEP
Brand: BOC Sciences
Rating: 5 (1 reviews)

Catalog Number	F04-0027
Category	Fluorescein FAM
Molecular Formula	C67H76N3O14P
Molecular Weight	1178.33

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Product Introduction

Fluorescein II CEP is a derivative of fluorescein, which is used in many fluorescent tracers. It can be attached to certain bioactive molecules, thus allowing biologists to target fluorophores to specific proteins or structures within cells.

Chemical Information
Product Specification
Application

Chemical Information

Synonyms	6-Fluorescein CE Phosphoramidite; Propanoic acid, 2,2-dimethyl-, 1,1'-[6-[8-[[bis(4-methoxyphenyl)phenylmethoxy]methyl]-10-(2-cyanoethoxy)-12-methyl-11-(1-methylethyl)-1-oxo-6,9-dioxa-2,11-diaza-10-phosphatridec-1-yl]-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3',6'-diyl] ester; 1,1'-[6-[8-[[Bis(4-methoxyphenyl)phenylmethoxy]methyl]-10-(2-cyanoethoxy)-12-methyl-11-(1-methylethyl)-1-oxo-6,9-dioxa-2,11-diaza-10-phosphatridec-1-yl]-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-3',6'-diyl] bis(2,2-dimethylpropanoate)
Purity	≥95%
IUPAC Name	6-((3-(3-(bis(4-methoxyphenyl)(phenyl)methoxy)-2-(((2-cyanoethoxy)(diisopropylamino)phosphaneyl)oxy)propoxy)propyl)carbamoyl)-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl bis(2,2-dimethylpropanoate)
SMILES	N#CCCOP(OC(COCCCNC(=O)C1=CC=C2C(=O)OC3(C4=CC=C(OC(=O)C(C)(C)C)C=C4OC5=CC(OC(=O)C(C)(C)C)=CC=C53)C2=C1)COC(C=6C=CC=CC6)(C7=CC=C(OC)C=C7)C8=CC=C(OC)C=C8)N(C(C)C)C(C)C
InChI	InChI=1S/C67H76N3O14P/c1-43(2)70(44(3)4)85(79-37-16-34-68)84-53(42-78-66(46-18-14-13-15-19-46,47-21-25-49(75-11)26-22-47)48-23-27-50(76-12)28-24-48)41-77-36-17-35-69-60(71)45-20-31-54-57(38-45)67(83-61(54)72)55-32-29-51(80-62(73)64(5,6)7)39-58(55)82-59-40-52(30-33-56(59)67)81-63(74)65(8,9)10/h13-15,18-33,38-40,43-44,53H,16-17,35-37,41-42H2,1-12H3,(H,69,71)
InChIKey	ZQNXVFAFTICSEX-UHFFFAOYSA-N
Boiling Point	1063.4±65.0 °C (Predicted)

Product Specification

Excitation	493
Emission	517
Storage	Store at 2-8 °C

Application

Fluorescein II CEP is a fluorescein-based fluorescent dye designed for conjugation workflows that benefit from a reactive handle and strong visible fluorescence. Its fluorescein core provides widely used excitation/emission for fluorescence imaging and labeling, while the CEP functionality supports attachment to biomolecules and other research materials to generate fluorescent conjugates for downstream assays.

1. Protein Labeling Workflows

Fluorescein II CEP is commonly used to prepare fluorescein-labeled proteins for fluorescence microscopy, fluorescence-based binding studies, and labeling controls in biochemical assays. Researchers use it to build fluorescent protein conjugates where the fluorescein reporting signal enables visualization of labeling distribution and tracking of conjugate behavior in solution or on assay surfaces. The conjugation-ready design supports practical reagent-to-conjugate preparation for antibody labeling, affinity reagent development, and general protein tagging in chemical biology.

2. Fluorescence Microscopy Tracing

Fluorescein II CEP is widely applied as a fluorescent labeling reagent for cellular and biomaterial imaging experiments that require a bright, visible-spectrum reporter. In microscopy workflows, it is used to generate fluorescent conjugates that can be incorporated into staining panels, used to trace biomolecule localization, or serve as a fluorescent tag for tracking interactions at the cell surface or within prepared biological samples. The fluorescein emission profile aligns well with standard fluorescence microscope filter sets used in routine imaging laboratories, supporting straightforward integration into established imaging protocols.

3. Fluorescent Bioassay Reagent Building

Fluorescein II CEP supports fluorescence assay development by enabling the preparation of fluorescein-tagged reagents used for readout in binding and detection formats. Assay developers use fluorescein conjugates to monitor reagent incorporation, track assay components, or generate fluorescent standards for plate-based measurements when a visible fluorescent signal is appropriate. This dye is frequently selected for building assay reagents where a robust fluorescein reporter provides a convenient optical handle for monitoring biochemical workflows in microplate readers and benchtop fluorescence instrumentation.

4. Bioconjugation for Labeled Polymers

Fluorescein II CEP is also used in biomaterials and polymer functionalization projects where fluorescent tagging of macromolecules enables imaging and characterization of functional materials. Researchers incorporate fluorescein-labeled polymer components into hydrogels, coatings, or other engineered constructs to visualize distribution, verify functionalization, and support fluorescence readouts in materials studies. The conjugation-ready fluorescein dye format helps translate fluorescent labeling from soluble biomolecules into polymer-based systems used in microscopy and fluorescence-based characterization.