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Product Introduction
Tetrazine (methyltetrazine) derivative of fluorescein, pure 5-isomer. Tetrazines are reactive heterodiene components for [4+2] inverse electron demand Diels-Alder reaction (ied-DA) with strained cycloalkenes (trans-cyclooctenes, cyclopropenes). This reaction is also called TCO (tetrazine-cyclooctene) ligation. The reaction is among the fastest bioconjugation reactions.
Chemical Information
Product Specification
Computed Properties
Synonyms |
5-isomer FAM tetrazine |
Purity |
NMR 1H, HPLC-MS (95%) |
IUPAC Name |
3',6'-dihydroxy-N-[[4-(6-methyl-1,2,4,5-tetrazin-3-yl)phenyl]methyl]-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxamide |
Canonical SMILES |
CC1=NN=C(N=N1)C2=CC=C(C=C2)CNC(=O)C3=CC4=C(C=C3)C5(C6=C(C=C(C=C6)O)OC7=C5C=CC(=C7)O)OC4=O |
InChI |
InChI=1S/C31H21N5O6/c1-16-33-35-28(36-34-16)18-4-2-17(3-5-18)15-32-29(39)19-6-9-23-22(12-19)30(40)42-31(23)24-10-7-20(37)13-26(24)41-27-14-21(38)8-11-25(27)31/h2-14,37-38H,15H2,1H3,(H,32,39) |
InChIKey |
VWBVWYWGTPSCBT-UHFFFAOYSA-N |
Solubility |
good in DMF, DMSO |
Appearance |
yellow solid |
ε, L⋅mol-1⋅cm-1 |
80000 |
Fluorescence Quantum Yield |
0.93 |
Excitation |
490 |
Emission |
513 |
Storage |
24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
XLogP3 |
2.9 |
Hydrogen Bond Donor Count |
3 |
Hydrogen Bond Acceptor Count |
10 |
Rotatable Bond Count |
4 |
Exact Mass |
559.14918341 g/mol |
Monoisotopic Mass |
559.14918341 g/mol |
Topological Polar Surface Area |
157Ų |
Heavy Atom Count |
42 |
Formal Charge |
0 |
Complexity |
974 |
Isotope Atom Count |
0 |
Defined Atom Stereocenter Count |
0 |
Undefined Atom Stereocenter Count |
0 |
Defined Bond Stereocenter Count |
0 |
Undefined Bond Stereocenter Count |
0 |
Covalently-Bonded Unit Count |
1 |
Compound Is Canonicalized |
Yes |