FAM phosphoramidite, 6-isomer (hydroxyprolinol) | CAS 959470-55-4

Name: FAM phosphoramidite, 6-isomer (hydroxyprolinol)
Brand: BOC Sciences
Rating: 5 (1 reviews)

Catalog Number	R10-0019
Category	Fluorescein FAM
Molecular Formula	C72H83N4O14P
Molecular Weight	1259.44

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Product Introduction

FAM phosphoramidite, 6-isomer (hydroxyprolinol) is a fluorescent labeling reagent used in oligonucleotide synthesis. It contains a 6-carboxyfluorescein (6-FAM) fluorophore attached to a hydroxyprolinol-based phosphoramidite moiety, enabling the incorporation of a fluorescent label at the 5' end of DNA or RNA sequences during automated solid-phase synthesis. The hydroxyprolinol linker provides a rigid, non-nucleosidic scaffold that may offer improved photophysical properties or reduced quenching compared to standard linkers. It is used in molecular biology applications including DNA sequencing, PCR fragment analysis, genotyping, and fluorescence resonance energy transfer (FRET) studies.

Chemical Information
Product Specification

Chemical Information

Synonyms	6-FAM Pro Phosphoramidite; 6-FAM Phosphoramidite (hydroxyprolinol); 6-Fluorescein phosphoramidite (hydroxyprolinol); 6-Carboxyfluorescein phosphoramidite (hydroxyprolinol); 6-((6-((2S,4R)-2-((Bis(4-methoxyphenyl)(phenyl)methoxy)methyl)-4-(((2-cyanoethoxy)(diisopropylamino)phosphaneyl)oxy)pyrrolidin-1-yl)-6-oxohexyl)carbamoyl)-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl bis(2,2-dimethylpropanoate)
Purity	≥95%
IUPAC Name	[6-[[6-[(2S,4R)-2-[[bis(4-methoxyphenyl)-phenylmethoxy]methyl]-4-[2-cyanoethoxy-[di(propan-2-yl)amino]phosphanyl]oxypyrrolidin-1-yl]-6-oxohexyl]carbamoyl]-6'-(2,2-dimethylpropanoyloxy)-3-oxospiro[2-benzofuran-1,9'-xanthene]-3'-yl] 2,2-dimethylpropanoate
SMILES	CC(C)N(C(C)C)P(OCCC#N)O[C@@H]1C[C@H](N(C1)C(=O)CCCCCNC(=O)C2=CC3=C(C=C2)C(=O)OC34C5=C(C=C(C=C5)OC(=O)C(C)(C)C)OC6=C4C=CC(=C6)OC(=O)C(C)(C)C)COC(C7=CC=CC=C7)(C8=CC=C(C=C8)OC)C9=CC=C(C=C9)OC
InChI	InChI=1S/C72H83N4O14P/c1-46(2)76(47(3)4)91(85-39-19-37-73)90-57-41-52(45-84-71(49-20-15-13-16-21-49,50-24-28-53(82-11)29-25-50)51-26-30-54(83-12)31-27-51)75(44-57)64(77)22-17-14-18-38-74-65(78)48-23-34-58-61(40-48)72(89-66(58)79)59-35-32-55(86-67(80)69(5,6)7)42-62(59)88-63-43-56(33-36-60(63)72)87-68(81)70(8,9)10/h13,15-16,20-21,23-36,40,42-43,46-47,52,57H,14,17-19,22,38-39,41,44-45H2,1-12H3,(H,74,78)/t52-,57+,91?/m0/s1
InChIKey	ZDVYQZZZQOKACR-YQZMZASNSA-N
Solubility	Soluble in DMSO
Appearance	Off-white solid