FAM hydrazide, 6-isomer | CAS 151890-73-2

FAM hydrazide, 6-isomer is a fluorescein (FAM) hydrazide derivative designed for covalent fluorescent labeling through hydrazone formation with aldehyde-containing targets. The fluorescein chromophore provides bright green emission for fluorescence imaging workflows, and the hydrazide functionality enables attachment to biomolecules and materials that can be presented with aldehyde groups. This reagent is commonly used to generate fluorescent conjugates for visualization and quantitative readouts in assay development, microscopy, and nucleic-acid or carbohydrate labeling strategies.

1. Aldehyde Biomolecule Labeling

FAM hydrazide, 6-isomer is used to fluorescently tag aldehyde-bearing biomolecules such as oxidized carbohydrates, glycoprotein-derived aldehydes, and other aldehyde-functionalized samples. Researchers employ it to prepare labeled conjugates for fluorescence microscopy and fluorescence-based bioassays where covalent attachment is required to reduce dye loss during washing. The hydrazide handle is particularly useful when the labeling target is generated by controlled oxidation steps that introduce aldehyde groups on proteins, polysaccharides, or surface-bound glycans.

2. Fluorescent Glycan And Carbohydrate Probes

FAM hydrazide, 6-isomer supports fluorescent glycomics-style workflows and carbohydrate labeling experiments in which glycans or glycoproteins are converted to aldehyde forms prior to staining. In practice, it is used to build fluorescent carbohydrate probes for tracking binding interactions, monitoring uptake or trafficking in cell-associated assays, and visualizing glycoconjugates in imaging studies. The fluorescein reporter enables straightforward detection on standard fluorescence microscope channels and plate readers when compatible excitation/emission settings are used.

3. Nucleic Acid And Oligo Labeling

FAM hydrazide, 6-isomer is applied to label nucleic-acid components when aldehyde groups are introduced on oligonucleotides or nucleic-acid-associated constructs. Fluorescently tagged oligos are commonly used in nucleic acid analysis workflows, including hybridization-based detection formats and fluorescence readouts in probe development. The covalent labeling approach helps maintain signal during hybridization and washing steps, which is valuable for reproducible imaging and quantitative measurements.

4. Biomaterial Surface Staining

FAM hydrazide, 6-isomer is used to fluorescently functionalize aldehyde-containing biomaterials and surfaces, enabling visualization of coatings, hydrogels, and scaffold modifications. Researchers incorporate it into materials characterization and imaging experiments to confirm surface functionalization and to track labeled regions in microscopy studies. This application is especially relevant for projects where aldehyde groups are introduced to a polymer or surface layer and a stable fluorescent tag is needed for downstream imaging and assay development.