FAM-dT Phosphoramidite | CAS 637330-26-8

FAM-dT Phosphoramidite is a fluorescein (FAM) labeled deoxythymidine phosphoramidite designed for automated oligonucleotide synthesis, enabling incorporation of a fluorescent dye directly into DNA probes and primers. This building block provides a convenient route to generate fluorescently tagged nucleic acids for fluorescence-based detection workflows, including hybridization assays and fluorescence readouts in nucleic acid analysis platforms.

1. Oligonucleotide Hybridization Probes

FAM-dT Phosphoramidite is used to prepare fluorescent DNA probes for hybridization-based assays where incorporation of a FAM-labeled nucleotide enables direct fluorescence readout of target binding. Researchers commonly employ FAM-labeled oligonucleotides in nucleic acid workflows such as probe-based screening, complementary strand detection, and assay development for fluorescence imaging or plate-based fluorescence measurements. Positioning the dye within the oligo allows probe designers to tune labeling density and placement to support robust signal generation during hybridization experiments.

2. Real-Time Nucleic Acid Assays

FAM-dT Phosphoramidite supports the construction of fluorescent nucleic acid reagents used in real-time amplification and monitoring formats, including primer/probe systems and fluorescence-tracking oligonucleotides. Molecular biology teams use FAM-labeled oligos to generate time-resolved fluorescence signals that report on oligonucleotide synthesis or target-dependent strand interactions. By enabling dye incorporation at the nucleotide level, this phosphoramidite helps streamline development of custom fluorescent oligo reagents for assay optimization, method transfer, and multiplexing strategies where defined fluorophore labeling is required.

3. Fluorescent DNA Standards And Controls

FAM-dT Phosphoramidite is also valuable for generating fluorescently labeled DNA standards, calibration materials, and assay controls used in nucleic acid quantification and method validation. Laboratories often synthesize defined FAM-tagged oligonucleotides to support instrument setup, gating and normalization in fluorescence-based workflows, and quality checks for probe performance across experimental runs. Because the dye is incorporated during oligonucleotide synthesis, these standards can be produced with consistent labeling patterns suitable for reproducible fluorescence reference measurements.

4. Fluorescent Labeling For Molecular Imaging

FAM-dT Phosphoramidite is employed to create fluorescently labeled DNA constructs used in molecular imaging and fluorescence microscopy experiments where nucleic acid localization or hybridization events are visualized. Cell and materials science groups use FAM-labeled oligonucleotides to track nucleic acid uptake, retention, or hybridization on complementary targets in imaging workflows. The phosphoramidite format enables straightforward incorporation of fluorescein into custom DNA sequences, supporting probe design for spatial studies where fluorescence microscopy readout is central.