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Fluorescein-5-maleimide | CAS 75350-46-8
| Catalog Number | F04-0027 |
| Category | Fluorescein FAM |
| Molecular Formula | C24H13NO7 |
| Molecular Weight | 427.4 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
Fluorescein-5-maleimide is a thiol reactive fluorescent probe used to label peptides and proteins.
Chemical Information
Product Specification
Application
| Synonyms | Fluorescein-5-maleimide (contains 2% N,N-Dimethylformamide at maximum);5-Maleimido-fluorescein; 5-MF; 1-(3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)-1H-pyrrole-2,5-dione |
| Purity | ≥90% |
| Canonical SMILES | C1=CC2=C(C=C1N3C(=O)C=CC3=O)C(=O)OC24C5=C(C=C(C=C5)O)OC6=C4C=CC(=C6)O |
| InChI | InChI=1S/C24H13NO7/c26-13-2-5-17-19(10-13)31-20-11-14(27)3-6-18(20)24(17)16-4-1-12(9-15(16)23(30)32-24)25-21(28)7-8-22(25)29/h1-11,26-27H |
| InChIKey | AYDAHOIUHVUJHQ-UHFFFAOYSA-N |
| Appearance | Orange to Brown Solid |
| MDL Number | MFCD00077345 |
| Excitation | 494 nm |
| Emission | 519 nm |
| Condition To Avoid | Light Sensitive,Air Sensitive,Moisture Sensitive,Heat Sensitive |
| Storage | Store at -20°C |
| Store Under Inert Gas | Store under inert gas |
Fluorescein-5-maleimide, a potent derivative of fluorescein renowned for its capacity to covalently bind to thiol groups, finds extensive utility across diverse scientific domains.
Protein Labeling: A common practice in scientific research involves employing Fluorescein-5-maleimide to label proteins for fluorescence-based analyses. This compound reacts with thiol groups on cysteine residues, enabling scientists to visualize and track protein localization and interactions. Such precise labeling plays a critical role in cellular imaging and unraveling the intricate dynamics at the molecular level.
Quantitative Assays: In the realm of biochemical assays, Fluorescein-5-maleimide emerges as a valuable tool for quantifying thiol groups present in proteins and other biomolecules. The fluorescence intensity exhibited directly mirrors the abundance of thiol groups, offering a precise means of measurement. This capability proves particularly advantageous in exploring redox biology and unraveling the varying oxidation states of proteins.
Live Cell Imaging: Fluorescein-5-maleimide stands out as a prized asset in live cell imaging scenarios owing to its ability to fluoresce under specific wavelengths. By labeling proteins or biomolecules within live cells, researchers gain the ability to monitor real-time biological processes. This technique plays a pivotal role in delving into cellular functions and deciphering molecular interactions within their natural milieu.
Diagnostic Applications: In the realm of diagnostics, Fluorescein-5-maleimide serves as a beacon of light, functioning as a fluorescent probe to detect thiol-containing compounds. Its exceptional sensitivity and specificity render it a fitting choice for various diagnostic assays, including those targeting disease biomarkers. By enhancing the accuracy and reliability of diagnostic tests, this compound elevates the standards of diagnostic assessments.
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