Fluorescein-5-maleimide | CAS 75350-46-8

Catalog Number	F04-0027
Category	Fluorescein FAM
Molecular Formula	C24H13NO7
Molecular Weight	427.4

Inquiry

* Please be kindly noted products are not for therapeutic use. We do not sell to patients.

Worldwide Delivery
Quality Assurance
24/7 Customer Service

Product Introduction

Fluorescein-5-maleimide is a thiol reactive fluorescent probe used to label peptides and proteins.

Chemical Information
Product Specification
Application

Synonyms	Fluorescein-5-maleimide (contains 2% N,N-Dimethylformamide at maximum);5-Maleimido-fluorescein; 5-MF; 1-(3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)-1H-pyrrole-2,5-dione
Purity	≥90%
Canonical SMILES	C1=CC2=C(C=C1N3C(=O)C=CC3=O)C(=O)OC24C5=C(C=C(C=C5)O)OC6=C4C=CC(=C6)O
InChI	InChI=1S/C24H13NO7/c26-13-2-5-17-19(10-13)31-20-11-14(27)3-6-18(20)24(17)16-4-1-12(9-15(16)23(30)32-24)25-21(28)7-8-22(25)29/h1-11,26-27H
InChIKey	AYDAHOIUHVUJHQ-UHFFFAOYSA-N
Appearance	Orange to Brown Solid
MDL Number	MFCD00077345

Excitation	494 nm
Emission	519 nm
Condition To Avoid	Light Sensitive,Air Sensitive,Moisture Sensitive,Heat Sensitive
Storage	Store at -20°C
Store Under Inert Gas	Store under inert gas

Fluorescein-5-maleimide, a potent derivative of fluorescein renowned for its capacity to covalently bind to thiol groups, finds extensive utility across diverse scientific domains.

Protein Labeling: A common practice in scientific research involves employing Fluorescein-5-maleimide to label proteins for fluorescence-based analyses. This compound reacts with thiol groups on cysteine residues, enabling scientists to visualize and track protein localization and interactions. Such precise labeling plays a critical role in cellular imaging and unraveling the intricate dynamics at the molecular level.

Quantitative Assays: In the realm of biochemical assays, Fluorescein-5-maleimide emerges as a valuable tool for quantifying thiol groups present in proteins and other biomolecules. The fluorescence intensity exhibited directly mirrors the abundance of thiol groups, offering a precise means of measurement. This capability proves particularly advantageous in exploring redox biology and unraveling the varying oxidation states of proteins.

Live Cell Imaging: Fluorescein-5-maleimide stands out as a prized asset in live cell imaging scenarios owing to its ability to fluoresce under specific wavelengths. By labeling proteins or biomolecules within live cells, researchers gain the ability to monitor real-time biological processes. This technique plays a pivotal role in delving into cellular functions and deciphering molecular interactions within their natural milieu.

Diagnostic Applications: In the realm of diagnostics, Fluorescein-5-maleimide serves as a beacon of light, functioning as a fluorescent probe to detect thiol-containing compounds. Its exceptional sensitivity and specificity render it a fitting choice for various diagnostic assays, including those targeting disease biomarkers. By enhancing the accuracy and reliability of diagnostic tests, this compound elevates the standards of diagnostic assessments.