6-Fluorescein phosphoramidite

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6-Fluorescein phosphoramidite

6-Fluorescein phosphoramidite | 204697-37-0

Catelog Number R10-0005
Category Fluorescein FAM
Molecular Formula C46H58N3O10P
Molecular Weight 843.94
Catalog Number Size Price Quantity
R10-0005 -- $--

Product Introduction

6-Fluorescein phosphoramidite is prepared from 6-carboxyfluorescein derivatives and does not contain 4,4'-dimethoxytrityl (DMT) groups. It can only be added once at the 5'end to terminate the synthesis.

Chemical Information

Synonyms FAM Phosphoramidite, 6-Isomer;6-fam phosphoramidite; FAM phosphoramidite, 6-isomer; DyLight FAM-6 CEP; 5'(6)-FAM; 5'-6-Fluorescein phosphoramidite
Purity ≥90%
IUPAC Name [6-[6-[2-cyanoethoxy-[di(propan-2-yl)amino]phosphanyl]oxyhexylcarbamoyl]-6'-(2,2-dimethylpropanoyloxy)-3-oxospiro[2-benzofuran-1,9'-xanthene]-3'-yl] 2,2-dimethylpropanoate
Canonical SMILES CC(C)N(C(C)C)P(OCCCCCCNC(=O)C1=CC2=C(C=C1)C(=O)OC23C4=C(C=C(C=C4)OC(=O)C(C)(C)C)OC5=C3C=CC(=C5)OC(=O)C(C)(C)C)OCCC#N
InChI InChI=1S/C46H58N3O10P/c1-29(2)49(30(3)4)60(55-25-15-22-47)54-24-14-12-11-13-23-48-40(50)31-16-19-34-37(26-31)46(59-41(34)51)35-20-17-32(56-42(52)44(5,6)7)27-38(35)58-39-28-33(18-21-36(39)46)57-43(53)45(8,9)10/h16-21,26-30H,11-15,23-25H2,1-10H3,(H,48,50)
InChI Key MGPYJVWEJNTXLC-UHFFFAOYSA-N
Solubility Good solubility in acetonitrile and DCM.
Appearance White to Off-white Solid
Boiling Point 850.6±65.0°C at 760 mmHg

Product Specification

ε, L⋅mol-1⋅cm-1 75000
Fluorescence Quantum Yield 0.9
Excitation 494
Emission 520
Storage Store at -20°C

Computed Properties

XLogP3 8.5
Hydrogen Bond Donor Count 1
Hydrogen Bond Acceptor Count 12
Rotatable Bond Count 21
Exact Mass 843.38598205 g/mol
Monoisotopic Mass 843.38598205 g/mol
Topological Polar Surface Area 163Ų
Heavy Atom Count 60
Formal Charge 0
Complexity 1470
Isotope Atom Count 0
Defined Atom Stereocenter Count 0
Undefined Atom Stereocenter Count 1
Defined Bond Stereocenter Count 0
Undefined Bond Stereocenter Count 0
Covalently-Bonded Unit Count 1
Compound Is Canonicalized Yes

Patents

Publication NumberTitlePriority Date
US-2020256857-A1 Universal histidine-tag binding compounds and methods of use thereof as fluorescent probes and sensors 2014-04-29
WO-2014120681-A2 Optical element for correcting color blindness 2013-01-29
EP-2739997-B1 Optical element for correcting color blindness 2011-08-05
US-2013032758-A1 Optical Element for Correcting Color Blindness 2011-08-05
US-2013033776-A1 Optical Element for Correcting Color Blindness 2011-08-05
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