FAM amine, 6-isomer | CAS 1313393-44-0

FAM amine, 6-isomer is a fluorescein-based fluorescent labeling reagent provided in an amine-reactive-ready form for attaching fluorescein to biomolecules and polymers. As a fluorescein derivative, it is commonly used as a visible-spectrum reporter in fluorescence microscopy and fluorescence-based assays, where its strong green emission supports sensitive visualization and readout in labeling workflows. The amine functionality enables convenient incorporation into conjugates used for tracking, quantification, and probe construction.

1. Fluorescent Antibody Labeling

FAM amine, 6-isomer is used in fluorescence labeling workflows to prepare antibody conjugates for immunofluorescence and binding studies. Researchers commonly incorporate this fluorescein reagent into antibody or protein labeling pipelines to generate fluorescent immunoreagents for imaging of target antigens in fixed-cell or fixed-tissue formats, as well as for fluorescence-based binding assays. The dye's widely used fluorescein emission profile aligns with standard filter sets and fluorescence plate reader configurations, making it a practical choice for assay development where green-channel detection is already established.

2. Fluorescence Microscopy Tracing

FAM amine, 6-isomer supports fluorescence microscopy experiments that require stable green labeling of biomolecules, peptides, or other research reagents used as tracers. In microscopy workflows, the reagent is frequently used to generate fluorescent conjugates for cellular localization studies, reagent uptake or transport studies, and colocalization experiments when paired with spectrally compatible dyes. Its fluorescein reporter character makes it straightforward to integrate into multi-color imaging panels designed around common excitation/emission detection schemes.

3. Oligonucleotide and Probe Conjugation

FAM amine, 6-isomer is employed to construct fluorescently labeled nucleic acid reagents used in nucleic acid analysis and hybridization-based assay development. In these workflows, the fluorescein tag enables visualization and quantitative readout of labeled oligonucleotides, primers, or probe constructs in fluorescence instrumentation such as gel imaging systems and fluorescence readers. The amine-functional labeling handle is particularly useful when building custom fluorescent probes for assay optimization, method development, and molecular biology research tool generation.

4. Flow Cytometry Labeling Reagents

FAM amine, 6-isomer is used to prepare fluorescein-labeled labeling reagents for flow cytometry panels that rely on green fluorescence detection. Researchers incorporate this dye into antibody conjugates or other fluorescent biomolecule conjugates to quantify binding to cell-surface markers, monitor labeling intensity distributions, and support gating strategies in multi-parameter experiments. The reagent's fluorescein emission characteristics align with commonly available cytometer configurations, enabling routine integration into established cytometry workflows for research-grade sample analysis.