FAM amine, 5-isomer | CAS 138589-19-2

FAM amine, 5-isomer is a fluorescein (FAM) derivative supplied as an amine-functionalized labeling building block for fluorescent conjugation workflows. As a reactive fluorophore, it is commonly used to introduce bright green fluorescence into biomolecules and imaging reagents, where amine-reactive coupling strategies enable attachment to proteins, peptides, and other amine-bearing targets. Its fluorescein emission is widely compatible with standard fluorescence microscopy and flow cytometry filter sets used for FITC/FAM-type dyes.

1. Protein Fluorescent Labeling

FAM amine, 5-isomer is frequently used by protein chemistry and antibody/affinity-reagent developers to create fluorescently labeled biomolecules for binding studies and fluorescence-based assays. Researchers typically incorporate the amine-functional fluorophore into labeling workflows where amine coupling chemistries generate stable, traceable conjugates for tracking protein localization, monitoring binding interactions, and visualizing biomolecular distributions in assay formats compatible with fluorescein detection.

2. Peptide And Biomolecule Conjugates

FAM amine, 5-isomer supports fluorescent tagging of peptides and other amine-containing biomolecules used in chemical biology and molecular imaging reagent development. In practice, teams use the fluorophore to prepare labeled ligands, probes, or standards for studying uptake, trafficking, and interaction patterns in fluorescence microscopy experiments, as well as for generating reference materials for assay development and method validation in fluorescence readouts.

3. Fluorescent Assay Reagent Development

FAM amine, 5-isomer is used to build fluorescence reporters and labeling reagents for assay development where fluorescein emission serves as the optical readout. Assay developers incorporate the dye into conjugates that can be tracked in endpoint or kinetic fluorescence formats, including workflows that require robust fluorescent labeling of biomolecular components such as capture reagents, detection reagents, or internal standards for fluorescence quantification.

4. Flow Cytometry Dye Conjugates

FAM amine, 5-isomer is commonly leveraged in flow cytometry workflows that require fluorescein-channel-compatible labeling of biomolecules. Researchers use it to generate fluorescent conjugates for staining and quantifying labeled targets in suspension, including experiments where amine-based coupling enables attachment of the fluorophore to proteins or other biomolecular binders used as staining reagents for cell-associated fluorescence readouts.