Cyanine7
Background
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Cyanine 7 dyes are a class of near-infrared cyanine fluorescent dyes with excitation and emission peaks around 750 and 770 nm, respectively. Their fluorescence is invisible to the naked eye and requires a near-infrared filter for microscopy. Certainly, cyanine 7 dyes are often used for in vivo imaging of small animals due to the fluorescence wavelength (Em: 773 nm) is just in the near-infrared window I region of body tissues. Biological tissue absorbs less light near the near-infrared; and relative to visible light, the near-infrared light has a longer decay length, which means stronger penetration within the tissue. Dyes with similar fluorescent properties include Alexa Fluor 750, DyLight 755, etc.
Characteristics of Cyanine 7 Dyes
Cyanine 7 dyes have poor water solubility, so organic co-solvents including DMF, DMSO and acetonitrile are used in the aqueous labeling reaction system. Such reagents can be used as fluorescent labels in the near-infrared spectral range when attachment to other molecules is not required. They are highly hydrophilic and water-soluble, enhance fluorescence yields in the near-infrared range, and have high molar extinction coefficients.
Application of Cyanine 7 Dyes
A variety of Cyanine 7 dyes are used for biomolecular labeling, fluorescence imaging, and other fluorescent bioassays, etc. Cyanine 7 dyes need to be displayed in the imaging software with the help of a CCD camera when observed under a microscope. In addition, near-infrared filters are rare, and many fluorescence microscopes (including confocal microscopes) are not equipped. But flow cytometers are often equipped with Cyanine 7 filter sets. But flow cytometers are often equipped with Cyanine 7 filter sets. Small animal imaging systems (eg, IVIS, Odyssey), near-infrared fluorescence scanners, and others are also equipped with Cyanine 7 filter sets. Cyanine 7 is a class of commonly used fluorescent dyes, which can be used to label nucleic acids, proteins, antibodies, polypeptides, nanoparticles, etc., and are common small animal imaging agents. Regardless of the structure, the quantum yields of Cyanine 7 are relatively low, generally <0.1. Low quantum yields are common to all NIR dyes, including NIR fluorescent proteins. The longer the wavelength of the dye, the lower the quantum yield.
Resources
- Hoechst Dyes: Definition, Structure, Mechanism and Applications
- Mastering the Spectrum: A Comprehensive Guide to Cy3 and Cy5 Dyes
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- Cyanine Dyes: Definition, Structure, Types and Uses
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- Cell Staining: Definition, Principles, Protocols, Dyes, and Uses
- Ion Imaging: Definition, Principles, Benefits, Dyes, and Uses
- Fluorescent Labeling: Definition, Principles, Types and Applications
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