Cyanine7 NHS ester | CAS 1432019-64-1

Cyanine7 NHS ester is a reactive cyanine dye designed for covalent fluorescent labeling through NHS ester chemistry, enabling stable attachment to primary amines on proteins, peptides, and other amine-containing biomolecules. Its far-red emission supports fluorescence imaging workflows that benefit from reduced background and convenient spectral separation from common visible fluorophores. The NHS-ester functionality makes it a widely used labeling reagent for preparing fluorescent conjugates for microscopy, imaging instrumentation, and fluorescence-based assay development.

1. Protein And Antibody Labeling

Cyanine7 NHS ester is routinely used by protein chemistry and chemical biology groups to generate far-red fluorescent conjugates for immunofluorescence, binding studies, and fluorescence readouts. Researchers label antibodies, antibody fragments, enzymes, and other amine-rich proteins to track biomolecular interactions, quantify binding in plate-based fluorescence assays, or visualize localization in cell-based experiments. The NHS ester reactivity supports straightforward conjugate preparation for downstream use in imaging systems that detect cyanine7 emission, including fluorescence microscopes and imaging plate readers.

2. Fluorescence Microscopy Staining

Cyanine7 NHS ester is commonly employed for fluorescence microscopy staining of fixed samples and labeled biomolecular targets where far-red signal provides strong contrast against cellular autofluorescence. Lab teams use the dye to visualize labeled proteins or protein complexes in immunostaining workflows, to map biomolecule distributions in microscopy experiments, and to create fluorescence-tagged reagents for co-localization studies with spectrally distinct markers. The covalent labeling approach helps maintain signal during washing steps typical of microscopy sample preparation.

3. Flow Cytometry Labeling

Cyanine7 NHS ester is used in flow cytometry workflows to produce fluorescently labeled protein reagents for cell-surface or intracellular staining strategies that rely on far-red detection channels. Researchers conjugate the dye to antibodies or other amine-containing binders to generate consistent fluorescent reagents for gating, population profiling, and multiplexing experiments where spectral separation from green and orange fluorophores is advantageous. Covalent attachment supports robust staining performance during typical sample handling and wash procedures.

4. Fluorescent Conjugate Standards

Cyanine7 NHS ester is also used in assay development and fluorescence method development to prepare fluorescent reference conjugates and calibration materials based on amine-reactive labeling. Instrumentation and assay teams generate dye-labeled proteins or peptides to validate detection workflows, optimize imaging/analysis parameters, and benchmark fluorescence signals in far-red channels. This labeling reagent format is especially useful when a reproducible, covalently attached fluorophore is required for method standardization across experiments.