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5-ROX, SE | 209734-74-7
| Catalog Number | R01-0027 |
| Category | Rhodamine |
| Molecular Formula | C37H33N3O7 |
| Molecular Weight | 631.68 |
| Catalog Number | Size | Price | Quantity |
|---|---|---|---|
| R01-0027 | -- | $-- |
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Product Introduction
ROX (rhodamine X) is a bright rhodamine dye for ROX/Texas Red channel. Carboxy rhodamines come as two isomers. This product is a derivative of pure 5-carboxy-ROX.
Chemical Information |
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|---|---|
| Synonyms | ROX NHS ester, 5-isomer;5-ROX, SE; 5(6)-ROX, SE; 5-(AND-6)-CARBOXY-X-RHODAMINE, SUCCINIMIDYL ESTER; 5-CARBOXY-X-RHODAMINE N-SUCCINIMIDYL ESTER; 5-CARBOXY-X-RHODAMINE, SUCCINIMIDYL ESTER |
| Purity | 95% |
| IUPAC Name | 5-(2,5-dioxopyrrolidin-1-yl)oxycarbonyl-2-(3-oxa-23-aza-9-azoniaheptacyclo[17.7.1.15,9.02,17.04,15.023,27.013,28]octacosa-1(27),2(17),4,9(28),13,15,18-heptaen-16-yl)benzoate |
| Canonical SMILES | C1CC2=C3C(=C4C(=C2)C(=C5C=C6CCC[N+]7=C6C(=C5O4)CCC7)C8=C(C=C(C=C8)C(=O)ON9C(=O)CCC9=O)C(=O)[O-])CCCN3C1 |
| InChI | InChI=1S/C37H33N3O7/c41-29-11-12-30(42)40(29)47-37(45)22-9-10-23(26(19-22)36(43)44)31-27-17-20-5-1-13-38-15-3-7-24(32(20)38)34(27)46-35-25-8-4-16-39-14-2-6-21(33(25)39)18-28(31)35/h9-10,17-19H,1-8,11-16H2 |
| InChI Key | BTTBJYLMDASSAS-UHFFFAOYSA-N |
| Solubility | good in DMF, DMSO |
| Appearance | Dark violet powder |
- Product Specification
- Application
| ε, L⋅mol-1⋅cm-1 | 93000 |
| Fluorescence Quantum Yield | 1.0 |
| Excitation | 570 |
| Emission | 591 |
| Storage | -20°C |
5-ROX, SE, a fluorescent dye commonly employed for labeling biomolecules, finds versatile applications across bioscience. Here are four key applications:
DNA Sequencing: As a crucial element in DNA sequencing, 5-ROX, SE acts as a vibrant fluorescent tag, facilitating the precise detection and interpretation of nucleotide sequences. Its intense fluorescence not only enhances the accuracy of automated sequencing technologies but also boosts the efficiency of genetic analyses by enabling clear visualization of genetic codes.
Real-Time PCR: In the realm of real-time PCR assays, 5-ROX, SE plays a pivotal role as a reference dye, ensuring the stability of fluorescence signals. By normalizing data and compensating for variations in sample quality and quantity, this dye guarantees the reliable quantification of target nucleic acids, a critical requirement for both diagnostic and research purposes.
Protein Labeling: Delving into fluorescence-based studies like FRET and flow cytometry, 5-ROX, SE emerges as a valuable tool for protein labeling. With its enduring fluorescence and superior quantum yield, this dye serves as an ideal agent for detecting and scrutinizing protein interactions, offering profound insights into the dynamic functions and interactions of proteins within cellular environments.
Cell Imaging: Unveiling its utility in cell biology, 5-ROX, SE takes center stage in staining and imaging cellular components. Its robust and consistent fluorescence properties make it a preferred choice for techniques like confocal microscopy, empowering researchers to visualize and monitor biomolecule distribution and mobility within live or fixed cells. This contribution fosters a deeper understanding of cellular structures and processes, unraveling the mysteries of cellular dynamics.
Applications of Fluorescent Probes & Dyes
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