Cyanine7 amine | CAS 1650635-41-8

Cyanine7 amine is a cyanine-based near-infrared fluorescent dye bearing an amine functional group for straightforward conjugation to biomolecules and materials. Its long-wavelength emission is commonly leveraged to reduce background autofluorescence in fluorescence imaging workflows, including microscopy and optical detection platforms. In practice, the dye's amine handle enables coupling into labeling reagents used for protein, peptide, and polymer conjugates, as well as fluorescent standards for assay development.

1. Protein And Antibody Labeling

Cyanine7 amine is frequently used by protein chemistry and fluorescence assay teams to generate near-infrared fluorescent conjugates for immunolabeling and binding studies. Researchers incorporate the amine functionality to attach the dye to lysine-containing proteins, antibodies, and antibody fragments, enabling visualization of target binding in fluorescence microscopy and optical readouts. This labeling strategy is particularly useful when experiments benefit from reduced cellular autofluorescence compared with visible fluorophores, supporting workflows such as target localization studies, binding kinetics experiments, and fluorescence-based characterization of affinity reagents.

2. Fluorescence Microscopy Tracing

Cyanine7 amine is applied in cellular and materials fluorescence microscopy where near-infrared emission improves signal readability against complex backgrounds. Imaging groups use dye-conjugated biomolecules to trace distributions of labeled proteins, peptides, or surface-associated ligands in fixed samples and engineered biomaterials. The amine functionality supports preparation of fluorescent conjugates that can be incorporated into staining or labeling protocols for tracking transport, localization, and co-localization with other fluorescent markers in multi-channel imaging experiments.

3. Biomaterial And Surface Functionalization

Cyanine7 amine is also used in biomaterials science to create fluorescently labeled surfaces and bulk materials for optical tracking and characterization. Materials researchers couple the dye to polymer backbones, coatings, or surface-reactive scaffolds to produce near-infrared fluorescent readouts for coating uniformity, adhesion studies, and material-biomolecule interaction experiments. The resulting Cyanine7-containing materials are commonly used in fluorescence imaging and optical quantification workflows to monitor labeling retention, spatial distribution, and binding of fluorescently tagged components on engineered substrates.

4. Fluorescent Conjugate Standards

Cyanine7 amine supports assay development and instrument setup by serving as a convenient starting material for preparing fluorescent reference conjugates and calibration standards. Fluorescence method developers use the dye to generate labeled reagents with defined optical behavior for validating channel performance, optimizing imaging or detection settings, and supporting comparative quantification across experiments. In these workflows, the amine handle is valuable for producing consistent dye-biomolecule conjugates that behave as practical standards in near-infrared fluorescence measurements.