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5(6)-Carboxyfluorescein diacetate | CAS 124387-19-5
| Catalog Number | A16-0025 |
| Category | pH Indicators |
| Molecular Formula | C25H16O9 |
| Molecular Weight | 460.4 |
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Product Introduction
5(6)-Carboxyfluorescein diacetate is a non-fluorescent precursor that undergoes enzymatic hydrolysis to release the fluorescent dye carboxyfluorescein within cells. This compound features a xanthene core structure, which upon de-esterification, exhibits strong green fluorescence with excitation and emission maxima suitable for a variety of bioimaging applications. It is commonly used in cell viability assays and intracellular tracking due to its ability to permeate cell membranes and generate fluorescence in response to esterase activity.
Chemical Information
Product Specification
Application
Chemical Information
| Synonyms | CFDA; 3',6'-bis(acetyloxy)-3-oxo-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-ar-carboxylic acid |
| Purity | ≥98% |
| pH | pKa 6.5 |
| Appearance | Solid Powder |
Product Specification
| Excitation | 494 nm |
| Emission | 521 nm |
| Storage | Store at -20°C |
Application
5(6)-Carboxyfluorescein diacetate, a versatile fluorescent dye with diverse bioscience applications, is at the forefront of cell research. Here are four key applications:
Cell Viability Assays: Employing 5(6)-Carboxyfluorescein diacetate in cell viability assays offers a window into the vitality and well-being of cells. Upon penetration of the cell membrane, this dye remains dormant until enzymatic cleavage by intracellular esterases triggers a vibrant green fluorescence. This distinctive feature enables researchers to discern between living and deceased cells, heightening the precision of cell viability assessments.
Cell Tracking and Migration Studies: This dye’s luminosity within live cells makes it indispensable for monitoring cell movements and migrations. By tagging cells with 5(6)-Carboxyfluorescein diacetate, scientists can track their trajectories and locations over time using advanced techniques like fluorescence microscopy or flow cytometry. Such applications prove vital in studies exploring cell migration, tissue regeneration, and immune responses.
Flow Cytometry Applications: In the realm of flow cytometry, 5(6)-Carboxyfluorescein diacetate plays a pivotal role in multi-parametric analyses of cell populations. Its fluorescence properties facilitate the quantitative assessment and segregation of cells based on their emission intensity. This utility is particularly advantageous in fields like immunology and hematology, enabling precise classification and profiling of distinct cell subsets.
Intracellular pH Measurement: Leveraging the pH-sensitive fluorescence of 5(6)-Carboxyfluorescein diacetate enables the precise measurement of intracellular pH levels. Upon uptake by the cell, the dye undergoes hydrolysis, transitioning into its fluorescent state, with intensity variations across different pH environments. This characteristic proves invaluable in research on cellular metabolism and ion transport mechanisms, shedding light on intricate biological processes.
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