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NPE-caged-HPTS | CAS 223759-19-1
| Catalog Number | A15-0001 |
| Category | pH Indicators |
| Molecular Formula | C24H14NO12Na3S3 |
| Molecular Weight | 673.53 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
NPE-caged-HPTS is a caged fluorescent pH indicator. It can release the fluorophore HPTS (pyranine) and can be used for photolysis calibration.
Chemical Information
Product Specification
Application
Computed Properties
| Synonyms | 8-Hydroxypyrene-1,3,6-tris-sulfonic acid-8-1-(2-nitrophenyl)ethyl ether |
| Purity | ≥97% by HPLC |
| IUPAC Name | trisodium;8-[1-(2-nitrophenyl)ethoxy]pyrene-1,3,6-trisulfonate |
| Canonical SMILES | CC(C1=CC=CC=C1[N+](=O)[O-])OC2=CC(=C3C=CC4=C(C=C(C5=C4C3=C2C=C5)S(=O)(=O)[O-])S(=O)(=O)[O-])S(=O)(=O)[O-].[Na+].[Na+].[Na+] |
| InChI | InChI=1S/C24H17NO12S3.3Na/c1-12(13-4-2-3-5-18(13)25(26)27)37-19-10-20(38(28,29)30)15-8-9-17-22(40(34,35)36)11-21(39(31,32)33)16-7-6-14(19)23(15)24(16)17;;;/h2-12H,1H3,(H,28,29,30)(H,31,32,33)(H,34,35,36);;;/q;3*+1/p-3 |
| InChIKey | CRGABPHUJQWBOR-UHFFFAOYSA-K |
| Storage | Store at -20°C |
NPE-caged-HPTS, a versatile chemical probe, finds utility in diverse experimental contexts for investigating dynamic biochemical processes. Here are four key applications of NPE-caged-HPTS:
Fluorescence Microscopy: Delving into the realm of live-cell imaging, NPE-caged-HPTS emerges as a pivotal tool for real-time exploration of cellular and subcellular dynamics. Upon being liberated from its caging group through UV-induced photolysis, the fluorescent dye HPTS comes to life. This liberation enables researchers to meticulously track intracellular pH fluctuations, ion movements, and various dynamic phenomena with exceptional spatial and temporal acuity.
Kinetic Studies: Embarking on a journey into the kinetics of swift biochemical reactions, scientists harness NPE-caged-HPTS to unravel the intricacies of rapid molecular transformations. By swiftly uncaging HPTS using light, researchers can synchronize reaction initiation and capture subsequent fluorescence alterations. This avenue proves particularly enlightening for scrutinizing enzyme catalysis, signal transduction cascades, and other rapid biochemical processes.
Drug Screening: Pioneering the domain of pharmacological inquiry, NPE-caged-HPTS serves as a crucial component in high-throughput screening endeavors aimed at assessing the impacts of potential pharmaceutical agents on cellular activities. Through the release of HPTS in the presence of test compounds, researchers witness fluorescence changes that elucidate shifts in cellular behavior indicative of drug-induced alterations. This methodology facilitates the identification of promising drug candidates endowed with desired bioactivities, paving the way for novel therapeutic avenues.
Calcium Signaling Studies: Unraveling the nuances of calcium dynamics within cellular milieus, NPE-caged-HPTS emerges as an indispensable asset for exploring the roles of calcium ions in diverse cellular functions. Post-photorelease from its caged state, HPTS steps into action, serving as a beacon for monitoring intracellular calcium oscillations through fluorescence variations. This application stands as a critical gateway to comprehending cellular responses to stimuli and the regulation of multiple physiological processes orchestrated by calcium signaling pathways, illuminating the intricate choreography of cellular communication.
| Hydrogen Bond Donor Count | 0 |
| Hydrogen Bond Acceptor Count | 12 |
| Rotatable Bond Count | 3 |
| Exact Mass | 672.93712125 g/mol |
| Monoisotopic Mass | 672.93712125 g/mol |
| Topological Polar Surface Area | 252Ų |
| Heavy Atom Count | 43 |
| Formal Charge | 0 |
| Complexity | 1260 |
| Isotope Atom Count | 0 |
| Defined Atom Stereocenter Count | 0 |
| Undefined Atom Stereocenter Count | 1 |
| Defined Bond Stereocenter Count | 0 |
| Undefined Bond Stereocenter Count | 0 |
| Covalently-Bonded Unit Count | 4 |
| Compound Is Canonicalized | Yes |
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