BCECF | CAS 85138-49-4

Catalog Number	A15-0004
Category	pH Indicators
Molecular Formula	C27H20O11
Molecular Weight	520.4

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Product Introduction

BCECF is a fluorescent cytoplasmic pH-sensitive indicator. pH is measured by the ratio of emission intensity.

Chemical Information
Product Specification
Application

Synonyms	2',7'-bis(carboxyethyl)-5(6)-Carboxyfluorescein; 5(or 6)-carboxy-3',6'-dihydroxy-3-oxo-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-dipropanoic acid
Purity	≥90%
Appearance	Solid Powder

Excitation	440-490 nm
Emission	535 nm
Storage	Store at -20°C

BCECF, a fluorescent dye with widespread usage in measuring pH in biological systems, boasts abundant applications in bioscience. Here are four key applications of BCECF:

Intracellular pH Measurement: BCECF serves as a pivotal tool in cell biology for gauging intracellular pH levels. By loading cells with BCECF and monitoring its fluorescence, researchers can probe fluctuations in pH triggered by diverse stimuli and treatments. This method is essential for unraveling the intricacies of cellular homeostasis, signaling pathways, and metabolic functions.

Diagnostics: In the realm of clinical diagnostics, BCECF emerges as a valuable resource for evaluating pH variations in patient samples. From detecting abnormalities in blood or urine pH, which may hint at underlying health conditions, to furnishing real-time and precise pH measurements, BCECF elevates the accuracy of diagnostics and enhances patient care.

Environmental Monitoring: Beyond biology, BCECF finds utility in environmental science for tracking pH levels in water bodies. By introducing BCECF into water samples, researchers can monitor pH fluctuations due to pollution or natural processes. This application holds paramount importance in evaluating ecosystem well-being and enacting measures for environmental preservation.

High-Throughput Screening: In the domain of drug discovery, BCECF plays a vital role in high-throughput screening assays designed to appraise the impact of compounds on cellular pH. By incorporating BCECF into these assays, researchers can swiftly assess the potential efficacy and toxicity of myriad drug candidates. This accelerates the drug development pipeline and expedites the identification of promising therapeutic interventions.