BCECF AM | 117464-70-7

Catalog Number	A15-0002
Category	pH Indicators
Molecular Formula	C42H40O21
Molecular Weight	880.7

Catalog Number	Size	Price	Quantity
A15-0002	--	$--

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Product Introduction

BCECF AM ester is a membrane-permeable fluorescent indicator for intracellular pH. It is modified by intracellular esterases to produce BCECF, a polar fluorescein derivative that is retained by cells.

Chemical Information
Product Specification
Application

Synonyms	BCECF Acetoxymethyl ester; BCECF-acetoxymethyl; 2',7'-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester; 3',6'-bis(acetyloxy)-5(or 6)-[[(acetyloxy)methoxy]carbonyl]-3-oxo-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-dipropanoic acid, 2',7'-bis[(acetyloxy)methyl] ester
Purity	≥95%
Canonical SMILES	CC(=O)OCOC1=C(C=C2C(=C1)OC3=CC(=C(C=C3C24C5=C(C=C(C=C5)C(=O)OCOC(=O)C)C(=O)O4)CCC(=O)OCOC(=O)C)OCOC(=O)C)CCC(=O)OCOC(=O)C
InChI	InChI=1S/C42H40O21/c1-22(43)52-17-57-34-15-36-32(13-27(34)7-10-38(48)59-19-54-24(3)45)42(31-9-6-29(12-30(31)41(51)63-42)40(50)61-21-56-26(5)47)33-14-28(8-11-39(49)60-20-55-25(4)46)35(16-37(33)62-36)58-18-53-23(2)44/h6,9,12-16H,7-8,10-11,17-21H2,1-5H3
InChI Key	NTECHUXHORNEGZ-UHFFFAOYSA-N

Excitation	440-490 nm
Emission	535 nm
Storage	Store at -20°C

BCECF AM, a renowned fluorescent dye in bioscience research, serves as a valuable tool for measuring intracellular pH. Here are four key applications of BCECF AM:

Intracellular pH Measurement: A cornerstone in cellular analysis, BCECF AM is extensively utilized to track pH fluctuations within living cells. By introducing the dye into cellular environments, researchers can harness fluorescence microscopy or flow cytometry to monitor pH dynamics in real-time. This application is indispensable for delving into studies concerning cellular metabolism, signal transduction, and ion transport mechanisms, offering a window into the intricate workings of cellular processes.

Drug Screening: At the forefront of pharmacological research, BCECF AM plays a pivotal role in high-throughput screening assays aimed at assessing the impacts of diverse compounds on intracellular pH levels. Variations in pH serve as indicators of drug efficacy, cytotoxicity, and various cellular responses, providing crucial insights into potential therapeutic agents and mechanisms of drug action. This method is a valuable asset in the realm of pharmaceutical research, offering a nuanced understanding of compound interactions within biological systems.

Live-cell Imaging: Elevating live-cell studies to a new level, BCECF AM is a go-to option for visualizing dynamic pH alterations across different cellular compartments. Researchers leverage advanced imaging techniques to chart pH gradients within organelles like lysosomes and mitochondria, shedding light on cellular physiology and the pivotal role pH plays in diverse biological processes. This approach enables a deeper exploration of cellular dynamics, paving the way for novel discoveries in cell biology.

Environmental Monitoring: Extending its reach beyond cellular realms, BCECF AM finds application in environmental science for scrutinizing the effects of pollutants and external factors on cellular viability and function. By examining shifts in intracellular pH, scientists can gauge the impact of toxic substances on cellular health, providing crucial insights for environmental toxicity assessments and ecological studies. This application underscores the versatility of BCECF AM in elucidating the interplay between environmental stressors and cellular responses, contributing to a comprehensive understanding of ecosystem dynamics.

Applications of Fluorescent Probes & Dyes