![{[Name]}](https://resource.bocsci.com/structure/151820-33-6.gif?v=2025011501)
![{[Name]}](https://resource.bocsci.com/structure/126208-13-7.gif?v=2025011501)
![{[Name]}](https://resource.bocsci.com/structure/5852-10-8.gif?v=2025011501)
![{[Name]}](https://resource.bocsci.com/structure/83619-73-2.gif?v=2025011501)
![{[Name]}](https://resource.bocsci.com/structure/128724-35-6.gif?v=2025011501)
![{[Name]}](https://resource.bocsci.com/structure/575-03-1.gif?v=2025011501)
Inquiry Basket
BCECF AM | CAS 117464-70-7
| Catalog Number | A15-0002 |
| Category | pH Indicators |
| Molecular Formula | C42H40O21 |
| Molecular Weight | 880.7 |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
BCECF AM ester is a membrane-permeable fluorescent indicator for intracellular pH. It is modified by intracellular esterases to produce BCECF, a polar fluorescein derivative that is retained by cells.
Chemical Information
Product Specification
Application
| Synonyms | BCECF Acetoxymethyl ester; BCECF-acetoxymethyl; 2',7'-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester; 3',6'-bis(acetyloxy)-5(or 6)-[[(acetyloxy)methoxy]carbonyl]-3-oxo-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-dipropanoic acid, 2',7'-bis[(acetyloxy)methyl] ester |
| Purity | ≥95% |
| Canonical SMILES | CC(=O)OCOC1=C(C=C2C(=C1)OC3=CC(=C(C=C3C24C5=C(C=C(C=C5)C(=O)OCOC(=O)C)C(=O)O4)CCC(=O)OCOC(=O)C)OCOC(=O)C)CCC(=O)OCOC(=O)C |
| InChI | InChI=1S/C42H40O21/c1-22(43)52-17-57-34-15-36-32(13-27(34)7-10-38(48)59-19-54-24(3)45)42(31-9-6-29(12-30(31)41(51)63-42)40(50)61-21-56-26(5)47)33-14-28(8-11-39(49)60-20-55-25(4)46)35(16-37(33)62-36)58-18-53-23(2)44/h6,9,12-16H,7-8,10-11,17-21H2,1-5H3 |
| InChIKey | NTECHUXHORNEGZ-UHFFFAOYSA-N |
| Excitation | 440-490 nm |
| Emission | 535 nm |
| Storage | Store at -20°C |
BCECF AM, a renowned fluorescent dye in bioscience research, serves as a valuable tool for measuring intracellular pH. Here are four key applications of BCECF AM:
Intracellular pH Measurement: A cornerstone in cellular analysis, BCECF AM is extensively utilized to track pH fluctuations within living cells. By introducing the dye into cellular environments, researchers can harness fluorescence microscopy or flow cytometry to monitor pH dynamics in real-time. This application is indispensable for delving into studies concerning cellular metabolism, signal transduction, and ion transport mechanisms, offering a window into the intricate workings of cellular processes.
Drug Screening: At the forefront of pharmacological research, BCECF AM plays a pivotal role in high-throughput screening assays aimed at assessing the impacts of diverse compounds on intracellular pH levels. Variations in pH serve as indicators of drug efficacy, cytotoxicity, and various cellular responses, providing crucial insights into potential therapeutic agents and mechanisms of drug action. This method is a valuable asset in the realm of pharmaceutical research, offering a nuanced understanding of compound interactions within biological systems.
Live-cell Imaging: Elevating live-cell studies to a new level, BCECF AM is a go-to option for visualizing dynamic pH alterations across different cellular compartments. Researchers leverage advanced imaging techniques to chart pH gradients within organelles like lysosomes and mitochondria, shedding light on cellular physiology and the pivotal role pH plays in diverse biological processes. This approach enables a deeper exploration of cellular dynamics, paving the way for novel discoveries in cell biology.
Environmental Monitoring: Extending its reach beyond cellular realms, BCECF AM finds application in environmental science for scrutinizing the effects of pollutants and external factors on cellular viability and function. By examining shifts in intracellular pH, scientists can gauge the impact of toxic substances on cellular health, providing crucial insights for environmental toxicity assessments and ecological studies. This application underscores the versatility of BCECF AM in elucidating the interplay between environmental stressors and cellular responses, contributing to a comprehensive understanding of ecosystem dynamics.
Recommended Services
Recommended Articles
- Hoechst Dyes: Definition, Structure, Mechanism and Applications
- Mastering the Spectrum: A Comprehensive Guide to Cy3 and Cy5 Dyes
- Fluorescent Probes: Definition, Structure, Types and Application
- Fluorescent Dyes: Definition, Mechanism, Types and Application
- Coumarin Dyes: Definition, Structure, Benefits, Synthesis and Uses
- BODIPY Dyes: Definition, Structure, Synthesis and Uses
- Cyanine Dyes: Definition, Structure, Types and Uses
- Fluorescein Dyes: Definition, Structure, Synthesis and Uses
- Rhodamine Dyes: Definition, Structure, Uses, Excitation and Emission
- Unlocking the Power of Fluorescence Imaging: A Comprehensive Guide
- Cell Imaging: Definitions, Systems, Protocols, Dyes, and Applications
- Lipid Staining: Definition, Principles, Methods, Dyes, and Uses
- Flow Cytometry: Definition, Principles, Protocols, Dyes, and Uses
- Nucleic Acid Staining: Definition, Principles, Dyes, Procedures, and Uses
- DNA Staining: Definition, Procedures, Benefits, Dyes and Uses
- Cell Staining: Definition, Principles, Protocols, Dyes, and Uses
- Ion Imaging: Definition, Principles, Benefits, Dyes, and Uses
- Fluorescent Labeling: Definition, Principles, Types and Applications
Recommended Products
Online Inquiry
