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5-Carboxyfluorescein N-succinimidyl ester | 92557-80-7
| Catelog Number | A16-0016 |
| Category | Fluorescein FAM |
| Molecular Formula | C25H15NO9 |
| Molecular Weight | 473.4 |
| Catalog Number | Size | Price | Quantity |
|---|---|---|---|
| A16-0016 | -- | $-- |
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Product Introduction
5-Carboxyfluorescein N-succinimidyl ester is a green fluorescent probe used for labeling nucleotides and peptides. It also can be used in apillary electrophoresis for fluorescent derivatization.
Chemical Information |
|
|---|---|
| Synonyms | 5-Carboxyfluorescein NHS ester; 5-Carboxyfluorescein-N-hydroxysuccinimide ester; 5-FAM SE; 5-FAM N-succinimidyl ester; 2,5-dioxo-1-pyrrolidinyl ester 3',6'-dihydroxy-3-oxo-spiro[isobenzofuran-1(3H), 9'-[9H]xanthene]-5-carboxylic acid |
| Purity | ≥95% |
| IUPAC Name | (2,5-dioxopyrrolidin-1-yl) 3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylate |
| Canonical SMILES | C1CC(=O)N(C1=O)OC(=O)C2=CC3=C(C=C2)C4(C5=C(C=C(C=C5)O)OC6=C4C=CC(=C6)O)OC3=O |
| InChI | InChI=1S/C25H15NO9/c27-13-2-5-17-19(10-13)33-20-11-14(28)3-6-18(20)25(17)16-4-1-12(9-15(16)24(32)34-25)23(31)35-26-21(29)7-8-22(26)30/h1-6,9-11,27-28H,7-8H2 |
| InChI Key | GECIDMICWWDIBO-UHFFFAOYSA-N |
| Appearance | Solid Powder |
Product Specification |
|
|---|---|
| Excitation | 491 nm |
| Emission | 518 nm |
| Storage | Store at -20°C |
| Signal | Warning |
| GHS Hazard Statements | H315 (100%): Causes skin irritation [Warning Skin corrosion/irritation] H319 (100%): Causes serious eye irritation [Warning Serious eye damage/eye irritation] H335 (100%): May cause respiratory irritation [Warning Specific target organ toxicity, single exposure; Respiratory tract irritation] |
| Precautionary Statement Codes | P261, P264, P264+P265, P271, P280, P302+P352, P304+P340, P305+P351+P338, P319, P321, P332+P317, P337+P317, P362+P364, P403+P233, P405, and P501 (The corresponding statement to each P-code can be found at the GHS Classification page.) |
Computed Properties | |
|---|---|
| XLogP3 | 2.4 |
| Hydrogen Bond Donor Count | 2 |
| Hydrogen Bond Acceptor Count | 9 |
| Rotatable Bond Count | 3 |
| Exact Mass | 473.07468106 g/mol |
| Monoisotopic Mass | 473.07468106 g/mol |
| Topological Polar Surface Area | 140Ų |
| Heavy Atom Count | 35 |
| Formal Charge | 0 |
| Complexity | 894 |
| Isotope Atom Count | 0 |
| Defined Atom Stereocenter Count | 0 |
| Undefined Atom Stereocenter Count | 0 |
| Defined Bond Stereocenter Count | 0 |
| Undefined Bond Stereocenter Count | 0 |
| Covalently-Bonded Unit Count | 1 |
| Compound Is Canonicalized | Yes |
Literatures
| PMID | Publication Date | Title | Journal |
|---|---|---|---|
| 9284 | 1976-09-01 | The role of lysine-41 in ribonuclease A studied by proton-magnetic-resonance spectroscopy of guanidinated ribonuclease A | European journal of biochemistry |
Patents
| Publication Number | Title | Priority Date |
|---|---|---|
| US-2023000980-A1 | Compositions and methods for using alternating electric fields to disrupt lipid capsules | 2021-06-30 |
| WO-2023275648-A1 | Compositions and methods for using alternating electric fields to disrupt lipid capsules | 2021-06-30 |
| WO-2022254340-A1 | Compositions and methods for treating with a combination of alternating electric fields and trastuzumab | 2021-06-01 |
| WO-2022232050-A1 | Compositions and methods for characterizing polynucleotide sequence alterations | 2021-04-26 |
| US-2022287957-A1 | Compositions and methods for using alternating electric fields to disrupt nanoparticles | 2021-03-12 |
Applications of Fluorescent Probes & Dyes
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